PTK2B antibody (pTyr402)

Catalog No. ABIN362251

Product Details anti-PTK2B Antibody

Target: PTK2B (PTK2B Protein tyrosine Kinase 2 beta (PTK2B))

Binding Specificity: pTyr402

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PTK2B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Specificity: The antibody detects endogenous level of Pyk2 only when phosphorylated at tyrosine 402.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pTyr402 (D-I-Y (p) -A-E) derived from Human Pyk2. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

References for anti-PTK2B antibody (ABIN362251)

Tomomura, Hasegawa, Suda, Sakagami, Tomomura et al.: "Serum calcium-decreasing factor, caldecrin, inhibits receptor activator of NF-?B ligand (RANKL)-mediated Ca2+ signaling and actin ring formation in mature osteoclasts via suppression of Src signaling ..." in: The Journal of biological chemistry, Vol. 287, Issue 22, pp. 17963-74, (2012) (PubMed).

Target Details for PTK2B

Target: PTK2B (PTK2B Protein tyrosine Kinase 2 beta (PTK2B))

Alternative Name: Pyk2 (PTK2B Products)

Synonyms: CADTK antibody, CAKB antibody, FADK2 antibody, FAK2 antibody, PKB antibody, PTK antibody, PYK2 antibody, RAFTK antibody, CAKbeta antibody, E430023O05Rik antibody, Raftk antibody, Pyk2 antibody, fak1b antibody, ptk2.2 antibody, ptk2b antibody, si:ch1073-355e8.1 antibody, protein tyrosine kinase 2 beta antibody, PTK2 protein tyrosine kinase 2 beta antibody, protein tyrosine kinase 2aa antibody, PTK2B antibody, Ptk2b antibody, ptk2aa antibody

Background: Involved in calcium induced regulation of ion channel and activation of the map kinase signaling pathway. May represent an important signaling intermediate between neuropeptide activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. Interacts with the SH2 domain of Grb2. May phosphorylate the voltage-gated potassium channel protein Kv1.2. Its activation is highly correlated with the stimulation of c-Jun N-terminal kinase activity. Involved in osmotic stress-dependent SNCA 'Tyr-125' phosphorylation.

Molecular Weight: 116 kDa

NCBI Accession: NP_004094

UniProt: Q14289

Pathways: EGFR Signaling Pathway, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Cell-Cell Junction Organization, Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGF Signaling