V-Rel Reticuloendotheliosis Viral Oncogene Homolog A, Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 3, P65 (RELA) (pSer529) antibody

Antigen: V-Rel Reticuloendotheliosis Viral Oncogene Homolog A, Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 3, P65 (RELA)

Synonyms: p50, KBF1, p105, EBP-1, MGC54151, NFKB-p50, NF-kappaB, NFKB-p105, NF-kappa-B, DKFZp686C01211, NF-kB, NF-KB1, Xrel1, nfkb3, xrela, MGC69459, NFKB1, kbf1, ebp-1, MGC86262, nfkb-p50, nf-kappaB, nfkb-p105, nf-kappa-b, RELA, p65, NFKB3, MGC131774

Epitope: pSer529

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Catalog no.: ABIN362253

Additional Information

Alternative name: NFkappaB-p65 (Phospho-Ser529)

Swiss-Prot: Q04206

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human NF-kappaB p65 around the phosphorylation site of serine 529 (L-L-S P -G-D).

Isotype: IgG  (Matching secondary antibodies)

Specificity: NFkappaB-p65 (Phospho-Ser529) (Human) antibody detects endogenous levels of NFkappaB-p65 proteinonly when phosphorylated at serine 529.

Synonyms: p50, KBF1, p105, EBP-1, MGC54151, NFKB-p50, NF-kappaB, NFKB-p105, NF-kappa-B, DKFZp686C01211, NF-kB, NF-KB1, Xrel1, nfkb3, xrela, MGC69459, NFKB1, kbf1, ebp-1, MGC86262, nfkb-p50, nf-kappaB, nfkb-p105, nf-kappa-b, RELA, p65, NFKB3, MGC131774

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 IF:1:100~1:200

Concentration: 50µg/50µl

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Buffer: Rabbit IgG in phosphate buffered saline (without Mg 2+ and Ca 2+ ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage: Store at -20°C. Avoid repeat freeze-thaw cycles.

Restrictions: For Research Use only