The antibody detects endogenous level of HSF1 only when phosphorylated at serine 303.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Immunogen
Peptide sequence around phosphorylation site of pSer303 (P-P-S (p) -P-P) derived from Human HSF1. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.