EIF2AK2 antibody (pThr451)

Catalog No. ABIN362373

Product Details anti-EIF2AK2 Antibody

Target: EIF2AK2 (Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2))

Binding Specificity: pThr451

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF2AK2 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Specificity: The antibody detects endogenous level of PKR only when phosphorylated at threonine 451.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pThr451 (K-G-T (p) -L-R) derived from Human PKR. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry: 1:50-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

References for anti-EIF2AK2 antibody (ABIN362373)

Laing, Wang, Briazova, Zhang, Wang, Zheng, Gow, Chen, Rajagopalan, Chen, Sun, Zhang: "Airborne particulate matter selectively activates endoplasmic reticulum stress response in the lung and liver tissues." in: American journal of physiology. Cell physiology, Vol. 299, Issue 4, pp. C736-49, (2010) (PubMed).

Target Details for EIF2AK2

Target: EIF2AK2 (Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2))

Alternative Name: PKR (EIF2AK2 Products)

Synonyms: PKR antibody, pkr antibody, EIF2AK2 antibody, DKFZp469K1934 antibody, EIF2AK1 antibody, PRKR antibody, 2310047A08Rik antibody, 4732414G15Rik antibody, AI467567 antibody, AI747578 antibody, Pkr antibody, Prkr antibody, Tik antibody, eukaryotic translation initiation factor 2 alpha kinase 2 antibody, eukaryotic translation initiation factor 2-alpha kinase 2 antibody, EIF2AK2 antibody, eif2ak2 antibody, Eif2ak2 antibody

Background: Following activation by double-stranded RNA in the presence of ATP, the kinase becomes autophosphorylated and can catalyze the phosphorylation of the translation initiation factor EIF2S1, which leads to an inhibition of the initiation of protein synthesis. Double-stranded RNA is generated during the course of a viral infection.

Molecular Weight: 68 kDa

NCBI Accession: NP_001129

UniProt: P19525

Pathways: DNA Damage Repair, ER-Nucleus Signaling, Hepatitis C