Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2) (pThr451) antibody

Details for Product No. ABIN362373
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Synonyms PKR, pkr, EIF2AK2, DKFZp469K1934, EIF2AK1, PRKR, 2310047A08Rik, 4732414G15Rik, AI467567, AI747578, Pkr, Prkr, Tik
(51), (50), (19), (13), (9), (7), (4), (4), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
(226), (55), (32)
(214), (17)
(5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (1), (1), (1)
Immunohistochemistry (IHC)
(148), (92), (82), (55), (40), (36), (19), (5), (2)
Pubmed 3 references available
Quantity 50 μL
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Catalog No. ABIN362373
198.00 $
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Immunogen Peptide sequence around phosphorylation site of pThr451 (K-G-T (p) -L-R) derived from Human PKR. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
Isotype IgG
Specificity The antibody detects endogenous level of PKR only when phosphorylated at threonine 451.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name PKR
Background Following activation by double-stranded RNA in the presence of ATP, the kinase becomes autophosphorylated and can catalyze the phosphorylation of the translation initiation factor EIF2S1, which leads to an inhibition of the initiation of protein synthesis. Double-stranded RNA is generated during the course of a viral infection.
Molecular Weight 68 kDa
NCBI Accession NP_001129
UniProt P19525
Research Area Signaling
Application Notes Immunohistochemistry: 1:50-1:100
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Background publications van Huizen, Martindale, Gorospe et al.: "P58IPK, a novel endoplasmic reticulum stress-inducible protein and potential negative regulator of eIF2alpha signaling." in: The Journal of biological chemistry, Vol. 278, Issue 18, pp. 15558-64, 2003 (PubMed).

Ruf, Lackey, Warudkar et al.: "Protection from interferon-induced apoptosis by Epstein-Barr virus small RNAs is not mediated by inhibition of PKR." in: Journal of virology, Vol. 79, Issue 23, pp. 14562-9, 2005 (PubMed).

Hebner, Wilson, Rader et al.: "Human papillomaviruses target the double-stranded RNA protein kinase pathway." in: The Journal of general virology, Vol. 87, Issue Pt 11, pp. 3183-93, 2006 (PubMed).

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