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AKT 1/2/3 antibody (pTyr312, pTyr315, pTyr316)

Reactivity: Human, Mouse, Rat IHC, IF Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN362433
  • Target See all AKT 1/2/3 products
    AKT 1/2/3
    Binding Specificity
    • 25
    • 23
    • 23
    • 23
    • 20
    • 20
    • 18
    • 18
    • 16
    • 16
    • 16
    • 7
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pTyr312, pTyr315, pTyr316
    Reactivity
    • 165
    • 153
    • 139
    • 46
    • 31
    • 28
    • 25
    • 15
    • 9
    • 6
    • 4
    • 4
    Human, Mouse, Rat
    Host
    • 163
    • 2
    Rabbit
    Clonality
    • 152
    • 13
    Polyclonal
    Conjugate
    • 42
    • 8
    • 8
    • 8
    • 8
    • 8
    • 8
    • 8
    • 8
    • 7
    • 7
    • 7
    • 7
    • 7
    • 7
    • 7
    • 2
    • 2
    • 2
    • 2
    • 2
    This AKT 1/2/3 antibody is un-conjugated
    Application
    • 148
    • 93
    • 92
    • 91
    • 36
    • 32
    • 29
    • 21
    • 9
    • 7
    • 6
    Immunohistochemistry (IHC), Immunofluorescence (IF)
    Specificity
    The antibody detects endogenous level of AKT1/AKT2/AKT3 only when phosphorylated at tyrosine 315/316/312.
    Purification
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.
    Immunogen
    Peptide sequence around phosphorylation site of pTyr315, 316, 312 (P-E-Y (p) -L-A) derived from Human AKT1, AKT2, AKT3. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
    Isotype
    IgG
  • Application Notes
    Western blotting: 1:500-1:1000
    Immunohistochemistry: 1:50-1:100
    Immunofluorescence: 1:100-1:200
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
  • Target
    AKT 1/2/3
    Alternative Name
    AKT1/AKT2/AKT3 (AKT 1/2/3 Products)
    Background
    General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase. /General protein kinase capable of phosphorylating several known proteins. IGF-1 leads to the activation of AKT3, which may play a role in regulating cell survival. Capable of phosphorylating several known proteins. Truncated isoform 2/PKB gamma 1 without the second serine phosphorylation site could still be stimulated but to a lesser extent.
    Molecular Weight
    60 kDa
    NCBI Accession
    NP_005456, NP_001617
    UniProt
    Q9Y243
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