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HDAC4 antibody (Histone Deacetylase 4) (pSer246)

Details for Product anti-HDAC4 Antibody No. ABIN362451, Supplier: Log in to see
Antigen
Epitope
pSer246
43
31
19
15
10
9
5
4
4
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
247
102
71
8
3
3
2
2
2
1
1
1
Host
Rabbit
216
37
2
Clonality
Polyclonal
Conjugate
This HDAC4 antibody is un-conjugated
10
9
9
4
4
4
3
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
223
83
50
28
26
17
13
10
6
5
2
1
1
1
1
Supplier
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Immunogen Peptide sequence around phosphorylation site of pSer246, 259, 220 (T-A-S (p) -EP) derived from Human HDAC4, HDAC5, HDAC9. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
Isotype IgG
Specificity The antibody detects endogenous level of HDAC4/HDAC5/HDAC9 only when phosphorylated at serine 246/259/220.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name HDAC4/HDAC5/HDAC9 (HDAC4 Antibody Abstract)
Background Histone Deacetylases (HDACs) are a group of enzymes closely related to sirtuins. They catalyze the removal of acetyl groups from lysine residues in histones and non-histone proteins, resulting in transcriptional repression. In general,they do not act autonomously but as components of large multiprotein complexes, such as pRb-E2F and mSin3A, that mediate important transcription regulatory pathways. There are three classes of HDACs, classes 1, 2 and 4, which are closely related Zn2+-dependent enzymes. HDACs are ubiquitously expressed and they can exist in the nucleus or cytosol. Their subcellular localization is effected by protein-protein interactions (for example HDAC-14.3.3 complexes are retained in the cytosol) and by the class to which they belong (class 1 HDACs are predominantly nuclear whilst class 2 HDACs shuttle between the nucleus and cytosol). HDACs have a role in cell growth arrest, differentiation and death and this has led to substantial interest in HDAC inhibitors as possible antineoplastic agents.
Molecular Weight 124 kDa
NCBI Accession NP_055522, NP_001015
UniProt Q9UKV0
Application Notes Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Background publications Vigushin, Coombes: "Targeted histone deacetylase inhibition for cancer therapy." in: Current cancer drug targets, Vol. 4, Issue 2, pp. 205-18, 2004 (PubMed).

Thiagalingam, Cheng, Lee et al.: "Histone deacetylases: unique players in shaping the epigenetic histone code." in: Annals of the New York Academy of Sciences, Vol. 983, pp. 84-100, 2003 (PubMed).

Marmorstein: "Structure and function of histone acetyltransferases." in: Cellular and molecular life sciences : CMLS, Vol. 58, Issue 5-6, pp. 693-703, 2001 (PubMed).

Cress, Seto: "Histone deacetylases, transcriptional control, and cancer." in: Journal of cellular physiology, Vol. 184, Issue 1, pp. 1-16, 2000 (PubMed).