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Mast/stem Cell Growth Factor Receptor (KIT) (pTyr936) antibody

Details for Product No. ABIN362466, Supplier: Log in to see
Antigen
  • Bs
  • CD117
  • Fdc
  • Gsfsco1
  • Gsfsco5
  • Gsfsow3
  • SCO1
  • SCO5
  • SOW3
  • Ssm
  • Tr-kit
  • W
  • c-KIT
  • C-Kit
  • PBT
  • SCFR
  • MGF
  • c-kit
Alternatives
anti-Human Mast/stem Cell Growth Factor Receptor antibody for Immunofluorescence
Epitope
pTyr936
84
65
39
36
32
24
22
22
16
14
13
13
13
13
13
12
12
10
10
9
9
9
9
9
9
9
9
9
9
8
8
8
8
8
8
8
8
8
8
8
8
8
7
7
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7
7
7
7
7
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
5
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
809
392
133
21
16
14
14
11
2
1
Host
Rabbit
631
228
207
4
1
1
Clonality
Polyclonal
Conjugate
Un-conjugated
87
72
70
66
48
40
15
13
11
11
11
8
8
8
8
8
7
5
4
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC)
370
345
345
174
133
111
92
60
41
33
19
13
11
11
8
4
4
3
2
2
2
2
2
2
Supplier
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Immunogen Peptide sequence around phosphorylation site of pTyr936 (H-I-Y (p) -S-N) derived from Human c-Kit. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
Isotype IgG
Specificity The antibody detects endogenous level of c-kit only when phosphorylated at tyrosine 936.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name c-kit (KIT Antibody Abstract)
Background This is the receptor for stem cell factor (mast cell growth factor). It has a tyrosine-protein kinase activity. Binding of the ligands leads to the autophosphorylation of KIT and its association with substrates such as phosphatidylinositol 3-kinase (Pi3K)
Molecular Weight 145 kDa
NCBI Accession NP_000213
UniProt P10721
Research Area Proliferation Markers
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Background publications Blume-Jensen, Jiang, Hyman et al.: "Kit/stem cell factor receptor-induced activation of phosphatidylinositol 3'-kinase is essential for male fertility." in: Nature genetics, Vol. 24, Issue 2, pp. 157-62, 2000 (PubMed).

Lowe: "Colipase stabilizes the lid domain of pancreatic triglyceride lipase." in: The Journal of biological chemistry, Vol. 272, Issue 1, pp. 9-12, 1997 (PubMed).

Martin, Suggs, Langley et al.: "Primary structure and functional expression of rat and human stem cell factor DNAs." in: Cell, Vol. 63, Issue 1, pp. 203-11, 1990 (PubMed).

Nocka, Tan, Chiu et al.: "Molecular bases of dominant negative and loss of function mutations at the murine c-kit/white spotting locus: W37, Wv, W41 and W." in: The EMBO journal, Vol. 9, Issue 6, pp. 1805-13, 1990 (PubMed).