KIT antibody (Mast/stem Cell Growth Factor Receptor) (pTyr936)

Details for Product anti-KIT Antibody No. ABIN362466, Supplier: Log in to see
Antigen
  • Bs
  • CD117
  • Fdc
  • Gsfsco1
  • Gsfsco5
  • Gsfsow3
  • SCO1
  • SCO5
  • SOW3
  • Ssm
  • Tr-kit
  • W
  • c-KIT
  • C-Kit
  • PBT
  • SCFR
  • MGF
  • c-kit
  • kit oncogene
  • v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
  • Kit
  • KIT
Alternatives
anti-Human KIT antibody for Cytometry by Time of Flight
Epitope
pTyr936
56
42
40
32
27
25
25
17
16
15
15
15
15
15
14
11
10
10
10
10
10
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10
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10
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7
7
7
7
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5
5
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4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
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1
Reactivity
Human
943
491
158
47
42
32
28
19
15
6
6
5
4
3
2
1
1
Host
Rabbit
687
284
242
4
2
1
Clonality
Polyclonal
Conjugate
This KIT antibody is un-conjugated
106
91
91
62
50
39
22
18
14
10
10
10
10
9
9
8
8
8
8
8
7
7
5
5
5
5
5
5
5
4
4
4
4
3
2
2
2
2
2
1
1
1
1
Application
Immunohistochemistry (IHC)
435
410
405
214
179
153
121
105
80
72
32
13
13
12
11
8
4
4
3
3
2
2
2
2
2
2
2
Options
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Immunogen Peptide sequence around phosphorylation site of pTyr936 (H-I-Y (p) -S-N) derived from Human c-Kit. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
Isotype IgG
Specificity The antibody detects endogenous level of c-kit only when phosphorylated at tyrosine 936.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name c-kit (KIT Antibody Abstract)
Background This is the receptor for stem cell factor (mast cell growth factor). It has a tyrosine-protein kinase activity. Binding of the ligands leads to the autophosphorylation of KIT and its association with substrates such as phosphatidylinositol 3-kinase (Pi3K)
Molecular Weight 145 kDa
NCBI Accession NP_000213
UniProt P10721
Research Area Proliferation Markers
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Sensory Perception of Sound, Stem Cell Maintenance, Production of Molecular Mediator of Immune Response, Regulation of long-term Neuronal Synaptic Plasticity
Application Notes Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Background publications Blume-Jensen, Jiang, Hyman, Lee, OGorman, Hunter: "Kit/stem cell factor receptor-induced activation of phosphatidylinositol 3'-kinase is essential for male fertility." in: Nature genetics, Vol. 24, Issue 2, pp. 157-62, 2000 (PubMed).

Lowe: "Colipase stabilizes the lid domain of pancreatic triglyceride lipase." in: The Journal of biological chemistry, Vol. 272, Issue 1, pp. 9-12, 1997 (PubMed).

Martin, Suggs, Langley, Lu, Ting, Okino, Morris, McNiece, Jacobsen, Mendiaz: "Primary structure and functional expression of rat and human stem cell factor DNAs." in: Cell, Vol. 63, Issue 1, pp. 203-11, 1990 (PubMed).

Nocka, Tan, Chiu, Chu, Ray, Traktman, Besmer: "Molecular bases of dominant negative and loss of function mutations at the murine c-kit/white spotting locus: W37, Wv, W41 and W." in: The EMBO journal, Vol. 9, Issue 6, pp. 1805-13, 1990 (PubMed).