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p65 antibody (Nuclear Factor-KB P65) (AA 534-538)

Details for Product anti-NFkBP65 Antibody No. ABIN362508, Supplier: Log in to see
Antigen
  • NFKB3
  • p65
  • NFkB
Alternatives
anti-Human p65 antibody for Western Blotting
Epitope
AA 534-538
103
77
67
53
49
47
44
42
40
37
33
25
22
16
15
15
14
13
13
10
10
9
9
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8
6
5
5
5
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4
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3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
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1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
1018
472
390
44
37
26
12
10
6
4
4
3
2
2
2
1
1
1
1
1
Host
Rabbit
945
79
13
7
2
Clonality
Polyclonal
Conjugate
This p65 antibody is un-conjugated
31
30
22
19
16
16
11
9
9
8
8
8
8
8
8
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
740
392
331
163
130
127
90
33
30
21
21
17
10
9
7
7
6
4
2
2
1
1
Supplier
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Immunogen Peptide sequence around AA 534-538 (F-S-S-I-A) derived from Human NFkB-p65. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
Isotype IgG
Specificity The antibody detects endogenous level of total NFkB-p65 protein.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Alternative Name NFkappaB-p65 (NFkBP65 Antibody Abstract)
Background NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex
Molecular Weight 65 kDa
NCBI Accession NP_001138
UniProt Q04206
Pathways NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Product cited in: Shah, Dhar, El Zahraa Mohammed et al.: "Prevention of acute kidney injury in a rodent model of cirrhosis following selective gut decontamination is associated with reduced renal TLR4 expression." in: Journal of hepatology, Vol. 56, Issue 5, pp. 1047-53, 2012 (PubMed).

Background publications Doyle, Jefferies, ONeill: "Bruton's tyrosine kinase is involved in p65-mediated transactivation and phosphorylation of p65 on serine 536 during NFkappaB activation by lipopolysaccharide." in: The Journal of biological chemistry, Vol. 280, Issue 25, pp. 23496-501, 2005 (PubMed).

Anwar, Fazal, Malik et al.: "RhoA/Rho-associated kinase pathway selectively regulates thrombin-induced intercellular adhesion molecule-1 expression in endothelial cells via activation of I kappa B kinase beta and phosphorylation ..." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 173, Issue 11, pp. 6965-72, 2004 (PubMed).

Baeuerle, Baltimore: "NF-kappa B: ten years after." in: Cell, Vol. 87, Issue 1, pp. 13-20, 1996 (PubMed).

Baeuerle, Henkel: "Function and activation of NF-kappa B in the immune system." in: Annual review of immunology, Vol. 12, pp. 141-79, 1994 (PubMed).

Haskill, Beg, Tompkins et al.: "Characterization of an immediate-early gene induced in adherent monocytes that encodes I kappa B-like activity." in: Cell, Vol. 65, Issue 7, pp. 1281-9, 1991 (PubMed).

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