HER2 antibody (V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian)) (AA 875-879)

Details for Product anti-ERBB2 Antibody No. ABIN362616, Supplier: Log in to see
Antigen
  • CD340
  • HER-2
  • HER-2/neu
  • HER2
  • MLN 19
  • NEU
  • NGL
  • TKR1
  • Erbb-2
  • Neu
  • c-erbB2
  • c-neu
  • mKIAA3023
  • v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog
  • v-erb-b2 avian erythroblastic leukemia viral oncogene 2
  • v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2
  • v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)
  • ERBB2
  • Erbb2
Alternatives
anti-Rat (Rattus) HER2 antibody for Cytometry by Time of Flight
Epitope
AA 875-879
104
85
72
71
57
54
54
48
46
35
26
24
22
22
20
20
20
20
20
20
20
20
20
20
19
19
19
18
17
15
13
13
13
12
11
8
8
8
8
8
7
7
6
6
6
5
5
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
1374
496
329
36
34
15
13
8
6
5
4
3
3
3
2
1
1
1
1
1
Host
Rabbit
1000
518
30
16
12
5
1
Clonality
Polyclonal
Conjugate
This HER2 antibody is un-conjugated
76
66
59
54
46
41
19
19
12
12
11
10
10
9
8
8
8
8
8
7
7
7
7
7
7
7
7
6
3
2
2
Application
Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
712
602
314
310
309
305
270
138
113
102
89
21
19
14
8
6
6
5
5
3
3
3
2
1
1
1
1
1
1
Options
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Immunogen Peptide sequence around AA 875-879 (T-E-Y-H-A) derived from Human HER2. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
Isotype IgG
Specificity The antibody detects endogenous level of total HER2 protein.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific immunogen.
Alternative Name HER2 (ERBB2 Antibody Abstract)
Background Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Not activated by EGF, TGF-a and amphiregulin.
Molecular Weight 185 kDa
UniProt P04626
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Skeletal Muscle Fiber Development
Application Notes Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Product cited in: Li, Bu, Wu, Wu, Huang: "The "HER2-PI3K/Akt-FASN Axis" regulated malignant phenotype of colorectal cancer cells." in: Lipids, Vol. 47, Issue 4, pp. 403-11, 2012 (PubMed).

Background publications Dittadi, Gion: "More about: prognostic importance of low c-erbB2 expression in breast tumors." in: Journal of the National Cancer Institute, Vol. 92, Issue 17, pp. 1443-4, 2000 (PubMed).

Klapper, Waterman, Sela, Yarden: "Tumor-inhibitory antibodies to HER-2/ErbB-2 may act by recruiting c-Cbl and enhancing ubiquitination of HER-2." in: Cancer research, Vol. 60, Issue 13, pp. 3384-8, 2000 (PubMed).

Muthuswamy, Gilman, Brugge: "Controlled dimerization of ErbB receptors provides evidence for differential signaling by homo- and heterodimers." in: Molecular and cellular biology, Vol. 19, Issue 10, pp. 6845-57, 2000 (PubMed).

Kwon, Bhattacharyya, Alberta, Giannobile, Cheon, Stiles, Pomeroy: "Activation of ErbB2 during wallerian degeneration of sciatic nerve." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 17, Issue 21, pp. 8293-9, 1997 (PubMed).

Qian, LeVea, Freeman, Dougall, Greene: "Heterodimerization of epidermal growth factor receptor and wild-type or kinase-deficient Neu: a mechanism of interreceptor kinase activation and transphosphorylation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 4, pp. 1500-4, 1994 (PubMed).