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p53 antibody (Tumor Protein P53) (AA 35-39)

Details for Product anti-TP53 Antibody No. ABIN362651, Supplier: Log in to see
Antigen
Alternatives
anti-Human p53 antibody for Immunohistochemistry (Paraffin-embedded Sections)
Epitope
AA 35-39
121
86
62
54
51
50
49
49
46
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43
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39
31
27
25
22
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17
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12
9
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3
3
3
3
2
2
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2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
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1
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1
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1
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1
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Reactivity
Human
1760
320
271
99
34
18
14
11
11
10
10
9
5
4
4
4
3
3
3
2
2
2
1
Host
Rabbit
1238
608
7
4
3
1
1
Clonality
Polyclonal
Conjugate
This p53 antibody is un-conjugated
54
45
31
28
20
20
17
15
15
14
14
14
14
14
14
2
2
2
2
2
2
2
2
2
2
1
Application
Western Blotting (WB)
1372
577
525
318
314
304
235
160
98
84
53
39
35
17
16
12
11
11
11
9
4
3
3
3
2
2
2
1
1
1
1
1
Supplier
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Immunogen Peptide sequence around AA 35-39 (L-P-S-Q-A) derived from Human p53. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
Isotype IgG
Specificity The antibody detects endogenous level of total p53 protein.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific immunogen.
Alternative Name p53 (TP53 Antibody Abstract)
Background P53 is a nuclear protein which plays an essential role in the regulation of cell cycle specifically in the transition from G0 to G1. It is found in very low levels in normal cells however in a variety of transformed cell lines in high amounts and believed to contribute to transformation and malignancy. The open reading frame of p53 is 393 amino acids long, with the central region (consisting of amino acids from about 100 to 300) containing the DNA-binding domain. This proteolysis-resistant core is flanked by a C-terminal end mediating oligomerization and an N-terminal end containing a strong transcription activation signal. p53 binds as a tetramer to a PBS (p53-Binding Site) and activates the expression of downstream genes that inhibit growth and/or invasion. p53 binds as a tetramer to a p53-binding site (PBS) and to activate the expression of adjacent genes that inhibit growth and/or invasion. Deletion of one or both p53 alleles reduces the expression of tetramers, resulting in decreased expression of the growth inhibitory genes
Molecular Weight 53 kDa
NCBI Accession NP_000537
UniProt P04637
Research Area Cancer, Cell Cycle, Transcription Factors, DNA/RNA, Apoptosis/Necrosis
Pathways p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling
Application Notes Western blotting: 1:500-1:1000
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Background publications Solomon, Pasupuleti, Xu et al.: "Inhibition of SIRT1 catalytic activity increases p53 acetylation but does not alter cell survival following DNA damage." in: Molecular and cellular biology, Vol. 26, Issue 1, pp. 28-38, 2005 (PubMed).

Cases, de Lorenzo: "The black cat/white cat principle of signal integration in bacterial promoters." in: The EMBO journal, Vol. 20, Issue 1-2, pp. 1-11, 2001 (PubMed).

Duboule: "Hox is in the hair: a break in colinearity?" in: Genes & development, Vol. 12, Issue 1, pp. 1-4, 1998 (PubMed).