Paxillin antibody (PXN) (AA 116-120)

Details for Product anti-PXN Antibody No. ABIN362686, Supplier: Log in to see
Antigen
  • CG18061
  • CG18576
  • CG31794
  • CT40481
  • CT42454
  • DPaxillin
  • DPxn
  • DPxn37
  • Dmel\\CG31794
  • Dpax
  • DpaxA
  • PDLP
  • dPax
  • pax
  • PXN
  • LOC100220858
  • AW108311
  • AW123232
  • Pax
  • wu:fw71f12
  • paxillin
  • Paxillin
  • paxillin-like
  • PXN
  • Pax
  • pxn
  • LOC100220858
  • Pxn
  • LOC100353305
Alternatives
anti-Human Paxillin antibody for Immunofluorescence (fixed cells)
Epitope
AA 116-120
63
52
27
20
17
15
14
14
13
10
9
8
7
6
4
4
4
4
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
393
209
198
32
12
11
10
8
5
5
4
3
1
1
1
1
1
1
Host
Rabbit
354
47
2
Clonality
Polyclonal
Conjugate
This Paxillin antibody is un-conjugated
7
7
7
5
5
5
5
5
5
5
5
5
4
4
2
2
2
2
2
Application
Immunohistochemistry (IHC), Western Blotting (WB)
315
105
88
64
59
47
31
21
8
5
4
4
2
1
1
1
1
Options
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Immunogen Peptide sequence around AA 116-120 (H-V-Y-S-F) derived from Human Paxillin. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
Isotype IgG
Specificity The antibody detects endogenous level of total Paxillin protein.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Alternative Name Paxillin (PXN Antibody Abstract)
Background Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).
Molecular Weight 68 kDa
NCBI Accession NP_001074
UniProt P49023
Research Area Cancer, Extracellular Matrix
Pathways MAPK Signaling, EGFR Signaling Pathway, Response to Growth Hormone Stimulus, Cell-Cell Junction Organization, Maintenance of Protein Location, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor
Application Notes Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Background publications Tao, Wollscheid, OBrien, Eng, Li, Bodenmiller, Watts, Hood, Aebersold: "Quantitative phosphoproteome analysis using a dendrimer conjugation chemistry and tandem mass spectrometry." in: Nature methods, Vol. 2, Issue 8, pp. 591-8, 2005 (PubMed).

Zhang, Wolf-Yadlin, Ross, Pappin, Rush, Lauffenburger, White: "Time-resolved mass spectrometry of tyrosine phosphorylation sites in the epidermal growth factor receptor signaling network reveals dynamic modules." in: Molecular & cellular proteomics : MCP, Vol. 4, Issue 9, pp. 1240-50, 2005 (PubMed).

Sanders, Basson: "p130cas but not paxillin is essential for Caco-2 intestinal epithelial cell spreading and migration on collagen IV." in: The Journal of biological chemistry, Vol. 280, Issue 25, pp. 23516-22, 2005 (PubMed).

Rush, Moritz, Lee, Guo, Goss, Spek, Zhang, Zha, Polakiewicz, Comb: "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells." in: Nature biotechnology, Vol. 23, Issue 1, pp. 94-101, 2005 (PubMed).