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Paxillin antibody (PXN) (AA 116-120)

Details for Product anti-PXN Antibody No. ABIN362686, Supplier: Log in to see
Antigen
  • CG18061
  • CG18576
  • CG31794
  • CT40481
  • CT42454
  • DPaxillin
  • DPxn
  • DPxn37
  • Dmel\\CG31794
  • Dpax
  • DpaxA
  • PDLP
  • dPax
  • pax
  • PXN
  • LOC100220858
  • AW108311
  • AW123232
  • Pax
  • wu:fw71f12
Alternatives
anti-Human Paxillin antibody for Western Blotting
Epitope
AA 116-120
60
49
25
24
20
17
14
13
9
7
7
7
7
6
6
5
5
4
4
4
4
4
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
366
165
151
25
6
3
3
2
1
1
1
1
1
Host
Rabbit
333
48
2
Clonality
Polyclonal
Conjugate
This Paxillin antibody is un-conjugated
7
7
7
7
5
5
5
5
5
5
5
5
2
2
2
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
290
100
91
58
47
46
26
22
8
8
4
2
2
1
1
1
Supplier
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Immunogen Peptide sequence around AA 116-120 (H-V-Y-S-F) derived from Human Paxillin. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
Isotype IgG
Specificity The antibody detects endogenous level of total Paxillin protein.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Alternative Name Paxillin (PXN Antibody Abstract)
Background Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).
Molecular Weight 68 kDa
NCBI Accession NP_001074
UniProt P49023
Research Area Cancer, Extracellular Matrix
Pathways MAPK Signaling, EGFR Signaling Pathway
Application Notes Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Background publications Tao, Wollscheid, OBrien et al.: "Quantitative phosphoproteome analysis using a dendrimer conjugation chemistry and tandem mass spectrometry." in: Nature methods, Vol. 2, Issue 8, pp. 591-8, 2005 (PubMed).

Zhang, Wolf-Yadlin, Ross et al.: "Time-resolved mass spectrometry of tyrosine phosphorylation sites in the epidermal growth factor receptor signaling network reveals dynamic modules." in: Molecular & cellular proteomics : MCP, Vol. 4, Issue 9, pp. 1240-50, 2005 (PubMed).

Sanders, Basson: "p130cas but not paxillin is essential for Caco-2 intestinal epithelial cell spreading and migration on collagen IV." in: The Journal of biological chemistry, Vol. 280, Issue 25, pp. 23516-22, 2005 (PubMed).

Rush, Moritz, Lee et al.: "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells." in: Nature biotechnology, Vol. 23, Issue 1, pp. 94-101, 2005 (PubMed).