Retinoblastoma 1 (RB1) (AA 793-797) antibody

Details for Product No. ABIN362687
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Antigen
Synonyms RB, pRb, OSRC, pp110, p105-Rb, Rb, Rb-1, P105-RB, PP110, RB11, zgc:154147, RB1
Epitope
AA 793-797
(32), (32), (29), (21), (20), (18), (17), (17), (16), (14), (10), (6), (4), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(332), (217), (203), (109), (85), (70), (48), (1), (1), (1)
Host
Rabbit
(287), (56), (2)
Clonality
Polyclonal
Conjugate
Un-conjugated
(10), (10), (10), (10), (10), (10), (10), (10), (10), (10), (10)
Application
Western Blotting (WB)
(209), (100), (94), (91), (84), (33), (25), (13), (10), (7), (2), (1), (1)
Pubmed 4 references available
Catalog no. ABIN362687
Quantity 50 µL
Price
165.00 $   Plus shipping costs $45.00
Shipping to United States (Change)
Availability Will be delivered in 2 to 3 Business Days

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Peptide sequence around AA 793-797 (P-S-S-P-L) derived from Human Rb. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
Isotype IgG
Specificity The antibody detects endogenous level of total Rb protein.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Alternative Name Rb
Background Key regulator of entry into cell division that acts as a tumor suppressor. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.
Molecular Weight 110 kDa
NCBI Accession NP_000312
UniProt P06400
Research Area Cancer, Transcription Factors
Application Notes Western blotting: 1:500-1:1000
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Background publications Knudsen, Wang: "Differential regulation of retinoblastoma protein function by specific Cdk phosphorylation sites." in: The Journal of biological chemistry, Vol. 271, Issue 14, pp. 8313-20, 1996 (PubMed).

Knudsen, Wang: "Dual mechanisms for the inhibition of E2F binding to RB by cyclin-dependent kinase-mediated RB phosphorylation." in: Molecular and cellular biology, Vol. 17, Issue 10, pp. 5771-83, 1997 (PubMed).

Chadee, Kyriakis: "MLK3 is required for mitogen activation of B-Raf, ERK and cell proliferation." in: Nature cell biology, Vol. 6, Issue 8, pp. 770-6, 2004 (PubMed).

Roesch, Becker, Meyer et al.: "Overexpression and hyperphosphorylation of retinoblastoma protein in the progression of malignant melanoma." in: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, Vol. 18, Issue 4, pp. 565-72, 2005 (PubMed).

Hosts (287), (56), (2)
Reactivities (332), (217), (203), (109), (85), (70), (48), (1), (1), (1)
Applications (209), (100), (94), (91), (84), (33), (25), (13), (10), (7), (2), (1), (1)
Conjugates (10), (10), (10), (10), (10), (10), (10), (10), (10), (10), (10)
Epitopes (32), (32), (29), (21), (20), (18), (17), (17), (16), (14), (10), (6), (4), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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