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Retinoblastoma 1 antibody (AA 778-782)

RB1 Reactivity: Human, Mouse, Rat WB, IHC Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN362691
  • Target See all Retinoblastoma 1 (RB1) Antibodies
    Retinoblastoma 1 (RB1)
    Binding Specificity
    • 32
    • 31
    • 29
    • 24
    • 24
    • 22
    • 20
    • 15
    • 11
    • 10
    • 10
    • 7
    • 6
    • 6
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 778-782
    Reactivity
    • 292
    • 113
    • 95
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    Human, Mouse, Rat
    Host
    • 253
    • 42
    Rabbit
    Clonality
    • 239
    • 56
    Polyclonal
    Conjugate
    • 148
    • 19
    • 17
    • 16
    • 11
    • 11
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Retinoblastoma 1 antibody is un-conjugated
    Application
    • 224
    • 132
    • 114
    • 80
    • 79
    • 42
    • 39
    • 36
    • 28
    • 19
    • 19
    • 9
    • 8
    • 6
    • 6
    • 2
    • 2
    Western Blotting (WB), Immunohistochemistry (IHC)
    Specificity
    The antibody detects endogenous level of taotal Rb protein.
    Purification
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
    Immunogen
    Peptide sequence around AA 778-782 (T-L-S-P-I) derived from Human Rb. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
    Isotype
    IgG
    Top Product
    Discover our top product RB1 Primary Antibody
  • Application Notes
    Western blotting: 1:500-1:1000
    Immunohistochemistry: 1:50-1:100
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
  • Braumüller, Wieder, Brenner, Aßmann, Hahn, Alkhaled, Schilbach, Essmann, Kneilling, Griessinger, Ranta, Ullrich, Mocikat, Braungart, Mehra, Fehrenbacher, Berdel, Niessner, Meier, van den Broek et al.: "T-helper-1-cell cytokines drive cancer into senescence. ..." in: Nature, Vol. 494, Issue 7437, pp. 361-5, (2013) (PubMed).

    Cai, Ye, Liu, Lu, Lu, Zhang, Wang, Cao: "Luteolin induced G2 phase cell cycle arrest and apoptosis on non-small cell lung cancer cells." in: Toxicology in vitro : an international journal published in association with BIBRA, Vol. 25, Issue 7, pp. 1385-91, (2011) (PubMed).

  • Target
    Retinoblastoma 1 (RB1)
    Alternative Name
    Rb (RB1 Products)
    Synonyms
    OSRC antibody, RB antibody, p105-Rb antibody, pRb antibody, pp110 antibody, Rb antibody, Rb-1 antibody, P105-RB antibody, PP110 antibody, RB11 antibody, zgc:154147 antibody, RB transcriptional corepressor 1 antibody, RB transcriptional corepressor like 1 antibody, retinoblastoma 1 antibody, RB1 antibody, RBL1 antibody, Rb1 antibody, rb1 antibody
    Background
    Key regulator of entry into cell division that acts as a tumor suppressor. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.
    Molecular Weight
    110 kDa
    NCBI Accession
    NP_000312
    UniProt
    P06400
    Pathways
    Cell Division Cycle, Intracellular Steroid Hormone Receptor Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Maintenance of Protein Location, Synthesis of DNA, Autophagy
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