PAK1/2/3 antibody (AA 421-425)

Catalog No. ABIN362788

Product Details anti-PAK1/2/3 Antibody

Target: PAK1/2/3 (P21-Activated Kinases 1/2/3 (PAK1/2/3))

Binding Specificity: AA 421-425

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAK1/2/3 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF)

Specificity: The antibody detects endogenous level of total PAK1/PAK2/PAK3 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific immunogen.

Immunogen: Peptide sequence around AA 421-425, 400-404, 419-423 (R-S-T-M-V) derived from Human PAK1, PAK2, PAK3. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Target Details for PAK1/2/3

Target: PAK1/2/3 (P21-Activated Kinases 1/2/3 (PAK1/2/3))

Alternative Name: PAK1/PAK2/PAK3 (PAK1/2/3 Products)

Synonyms: X-PAK1 antibody, XPak1 antibody, pak antibody, pak-1 antibody, pakalpha antibody, p21 protein (Cdc42/Rac)-activated kinase 1 S homeolog antibody, p21 (RAC1) activated kinase 1 antibody, pak1.S antibody, PAK1 antibody

Background: The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.

NCBI Accession: NP_001121, NP_002568

UniProt: O75914