Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3) (AA 1175-1179) antibody

Details for Product No. ABIN362789
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Antigen
Synonyms NOS3, ECNOS, eNOS, NOS, EC-NOS, NOSIII, cNOS, 2310065A03Rik, Nos-3, ecNOS, eNos, ENOS
Epitope
AA 1175-1179
(32), (27), (25), (18), (17), (16), (15), (15), (15), (13), (10), (10), (8), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(315), (202), (198), (80), (69), (65), (37), (3), (2), (1), (1), (1)
Host
Rabbit
(334), (22)
Clonality
Polyclonal
Conjugate
Un-conjugated
(17), (12), (12), (8), (8), (8), (8), (8), (8), (8), (8), (3), (3), (3)
Application
Western Blotting (WB), Immunofluorescence (IF)
(223), (138), (80), (70), (47), (30), (29), (12), (12), (10), (9), (2), (1), (1), (1)
Pubmed 3 references available
Quantity 50 µL
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Catalog No. ABIN362789
132.00 $
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Immunogen Peptide sequence around AA 1175-1179 (T-Q-S-F-S) derived from Human eNOS. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
Isotype IgG
Specificity The antibody detects endogenous level of total eNOS protein.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific immunogen.
Alternative Name eNOS
Background Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
Molecular Weight 140 kDa
NCBI Accession NP_000594
UniProt P29474
Research Area Cancer, Enzymes, Metabolism, Inflammation
Application Notes Western blotting: 1:500-1:1000
Immunofluorescence: 1:100-1:200
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Background publications Wadman: "GM advisory panel is slanted, say critics." in: Nature, Vol. 399, Issue 6731, pp. 7, 1999 (PubMed).

Harris, Ju, Venema et al.: "Reciprocal phosphorylation and regulation of endothelial nitric-oxide synthase in response to bradykinin stimulation." in: The Journal of biological chemistry, Vol. 276, Issue 19, pp. 16587-91, 2001 (PubMed).

Thomas, Chen, Keaney: "Hydrogen peroxide activates endothelial nitric-oxide synthase through coordinated phosphorylation and dephosphorylation via a phosphoinositide 3-kinase-dependent signaling pathway." in: The Journal of biological chemistry, Vol. 277, Issue 8, pp. 6017-24, 2002 (PubMed).

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