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ZAP70 antibody (zeta-Chain (TCR) Associated Protein Kinase 70kDa) (AA 317-321)

Details for Product anti-ZAP70 Antibody No. ABIN362795, Supplier: Log in to see
Antigen
Alternatives
anti-Human ZAP70 antibody for Western Blotting
Epitope
AA 317-321
50
47
44
25
22
21
21
20
15
15
15
14
12
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
454
171
119
4
4
3
2
2
2
Host
Rabbit
295
140
15
8
2
Clonality
Polyclonal
Conjugate
This ZAP70 antibody is un-conjugated
29
21
16
14
10
7
5
5
4
4
4
4
4
4
4
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
276
162
151
81
62
44
39
38
9
7
5
4
4
3
2
Supplier
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Immunogen Peptide sequence around AA 317-321 (S-P-Y-S-D) derived from Human ZAP70. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
Isotype IgG
Specificity The antibody detects endogenous level of total Zap-70 protein.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Alternative Name Zap-70 (ZAP70 Antibody Abstract)
Background ZAP70 is a 70-kD tyrosine phosphoprotein that associates with the zeta chain and undergoes tyrosine phosphorylation following TCR stimulation. The ZAP70 gene is expressed in T- and natural KILLER cells. Protein-Tyrosine Kinases (PTKs) play an integral role in T-cell activation. Stimulation of the T-cell antigen receptor results in tyrosine phosphorylation of a number of cellular substrates. One of these is the TCR-zeta chain, which can mediate the transduction of extracellular stimuli into cellular effector functions
Molecular Weight 70 kDa
UniProt P43403
Research Area Signaling, Tags/Labels, Immunology, Cancer
Pathways TCR Signaling
Application Notes Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Background publications Adjali, Marodon, Steinberg et al.: "In vivo correction of ZAP-70 immunodeficiency by intrathymic gene transfer." in: The Journal of clinical investigation, Vol. 115, Issue 8, pp. 2287-95, 2005 (PubMed).

Salomon, Ficarro, Brill et al.: "Profiling of tyrosine phosphorylation pathways in human cells using mass spectrometry." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 2, pp. 443-8, 2003 (PubMed).

Ku, Yablonski, Manser et al.: "A PAK1-PIX-PKL complex is activated by the T-cell receptor independent of Nck, Slp-76 and LAT." in: The EMBO journal, Vol. 20, Issue 3, pp. 457-65, 2001 (PubMed).

Tang, Sawasdikosol, Chang et al.: "SLAP, a dimeric adapter protein, plays a functional role in T cell receptor signaling." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 17, pp. 9775-80, 1999 (PubMed).

Williams, Irvin, Sutor et al.: "Phosphorylation of Tyr319 in ZAP-70 is required for T-cell antigen receptor-dependent phospholipase C-gamma1 and Ras activation." in: The EMBO journal, Vol. 18, Issue 7, pp. 1832-44, 1999 (PubMed).

Zhao, Weiss: "Enhancement of lymphocyte responsiveness by a gain-of-function mutation of ZAP-70." in: Molecular and cellular biology, Vol. 16, Issue 12, pp. 6765-74, 1997 (PubMed).