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ZAP70 antibody (zeta-Chain (TCR) Associated Protein Kinase 70kDa) (AA 491-495)

Details for Product anti-ZAP70 Antibody No. ABIN362798, Supplier: Log in to see
Antigen
  • zap70
  • MGC80670
  • MGC88962
  • ZAP70
  • 70kDa
  • AI327364
  • Srk
  • TZK
  • ZAP-70
  • mrtle
  • mur
  • SRK
  • STCD
  • STD
Alternatives
anti-Human ZAP70 antibody for Western Blotting
Epitope
AA 491-495
50
47
46
25
22
21
21
20
15
15
15
14
12
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
456
170
118
4
4
3
2
2
2
Host
Rabbit
295
141
15
8
3
Clonality
Polyclonal
Conjugate
This ZAP70 antibody is un-conjugated
27
20
16
14
10
7
5
5
4
4
4
4
4
4
4
1
Application
Immunohistochemistry (IHC)
279
162
150
82
62
44
39
38
10
7
5
4
4
3
2
Supplier
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Immunogen Peptide sequence around AA 491-495 (S-Y-Y-T-A) derived from Human ZAP70. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
Isotype IgG
Specificity The antibody detects endogenous level of total Zap-70 protein.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Alternative Name Zap-70 (ZAP70 Antibody Abstract)
Background ZAP70 is a 70-kD tyrosine phosphoprotein that associates with the zeta chain and undergoes tyrosine phosphorylation following TCR stimulation. The ZAP70 gene is expressed in T- and natural KILLER cells. Protein-Tyrosine Kinases (PTKs) play an integral role in T-cell activation. Stimulation of the T-cell antigen receptor results in tyrosine phosphorylation of a number of cellular substrates. One of these is the TCR-zeta chain, which can mediate the transduction of extracellular stimuli into cellular effector functions
Molecular Weight 70 kDa
UniProt P43403
Research Area Signaling, Tags/Labels, Immunology, Cancer
Pathways TCR Signaling
Application Notes Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Background publications Salomon, Ficarro, Brill et al.: "Profiling of tyrosine phosphorylation pathways in human cells using mass spectrometry." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 2, pp. 443-8, 2003 (PubMed).

Kong, Dalton, Bubeck Wardenburg et al.: "Distinct tyrosine phosphorylation sites in ZAP-70 mediate activation and negative regulation of antigen receptor function." in: Molecular and cellular biology, Vol. 16, Issue 9, pp. 5026-35, 1996 (PubMed).

Chan, Dalton, Johnson et al.: "Activation of ZAP-70 kinase activity by phosphorylation of tyrosine 493 is required for lymphocyte antigen receptor function." in: The EMBO journal, Vol. 14, Issue 11, pp. 2499-508, 1995 (PubMed).