STAT1 antibody (Signal Transducer and Activator of Transcription 1, 91kDa) (AA 725-729)

Details for Product anti-STAT1 Antibody No. ABIN362804, Supplier: Log in to see
Antigen
  • IMUP
  • H2RSP
  • IMUP-1
  • IMUP-2
  • 2010005J02Rik
  • AA408197
  • DD6G4-4
  • CANDF7
  • ISGF-3
  • STAT91
  • STAT4
  • XStat1
  • STAT
  • STAT1
  • dZ182H3.3
  • si:by134g18.2
  • si:by51f19.1
  • si:dz182h3.3
  • si:dz199m19.1
  • si:dz73o4.2
  • signal transducer and activator of transcription 1
  • RIKEN cDNA 2200002D01 gene
  • signal transducer and activator of transcription 1, 91kDa
  • signal transducer and activator of transcription 1 L homeolog
  • signal transducer and activator of transcription 4
  • STAT1
  • 2200002D01Rik
  • Stat1
  • stat1.L
  • stat4
Alternatives
anti-Human STAT1 antibody for Immunoprecipitation
Epitope
AA 725-729
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81
61
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1
1
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1
1
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Reactivity
Human, Mouse (Murine), Rat (Rattus)
614
295
240
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27
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18
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Host
Rabbit
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3
1
Clonality
Polyclonal
Conjugate
This STAT1 antibody is un-conjugated
18
18
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Application
Immunohistochemistry (IHC), Western Blotting (WB)
535
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106
54
52
31
24
14
10
5
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2
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Options
Supplier
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Immunogen Peptide sequence around AA 725-729 (P-M-S-P-E) derived from Human STAT1. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
Isotype IgG
Specificity The antibody detects endogenous level of total STAT1 protein.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Alternative Name STAT1 (STAT1 Antibody Abstract)
Background Signal transducer and activator of transcription that mediates signaling by interferons (IFNs). Following type I IFN (IFN-a and IFN-beta) binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state.
Molecular Weight 91 kDa
UniProt P42224
Research Area Cancer, Transcription Factors, Signaling, Receptors, Inflammation
Pathways JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Response to Growth Hormone Stimulus, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Hepatitis C, CXCR4-mediated Signaling Events
Application Notes Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Product cited in: Cheng, Wang, Xia, Yan, Tang, Chen, Zhang, Liu, Kong, Sjöberg, Folco, Libby, Liao, Shi: "A guanidine-rich regulatory oligodeoxynucleotide improves type-2 diabetes in obese mice by blocking T-cell differentiation." in: EMBO molecular medicine, Vol. 4, Issue 10, pp. 1112-25, 2012 (PubMed).

Background publications Strausberg, Feingold, Grouse, Derge, Klausner, Collins, Wagner, Shenmen, Schuler, Altschul, Zeeberg, Buetow, Schaefer, Bhat, Hopkins, Jordan, Moore, Max, Wang, Hsieh, Diatchenko, Marusina, Farmer et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. ..." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, 2002 (PubMed).

Yuan, Shioya, Ishiko, Sun, Gu, Huang, Lu, Kharbanda, Weichselbaum, Kufe: "p73 is regulated by tyrosine kinase c-Abl in the apoptotic response to DNA damage." in: Nature, Vol. 399, Issue 6738, pp. 814-7, 1999 (PubMed).

Quelle, Thierfelder, Witthuhn, Tang, Cohen, Ihle: "Phosphorylation and activation of the DNA binding activity of purified Stat1 by the Janus protein-tyrosine kinases and the epidermal growth factor receptor." in: The Journal of biological chemistry, Vol. 270, Issue 35, pp. 20775-80, 1995 (PubMed).

Schindler, Fu, Improta, Aebersold, Darnell: "Proteins of transcription factor ISGF-3: one gene encodes the 91-and 84-kDa ISGF-3 proteins that are activated by interferon alpha." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 16, pp. 7836-9, 1992 (PubMed).

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