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EGFR antibody (Epidermal Growth Factor Receptor) (AA 867-871)

Details for Product anti-EGFR Antibody No. ABIN362883, Supplier: Log in to see
Antigen
Alternatives
anti-Human EGFR antibody for Western Blotting
Epitope
AA 867-871
127
57
55
52
51
49
48
45
45
44
44
33
32
30
30
25
23
23
23
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19
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13
11
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5
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4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
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1
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1
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1
1
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1
1
1
1
1
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1
1
1
1
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1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
1705
666
594
21
14
14
13
11
7
7
4
2
1
1
1
Host
Rabbit
1268
393
36
25
4
2
Clonality
Polyclonal
Conjugate
This EGFR antibody is un-conjugated
74
57
45
35
26
25
25
23
21
21
21
21
21
21
20
2
2
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
1103
500
380
240
236
205
175
142
86
60
55
54
25
11
9
5
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Supplier
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Immunogen Peptide sequence around AA 867-871 (K-E-Y-H-A) derived from Human EGFR. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
Isotype IgG
Specificity The antibody detects endogenous level of total EGFR protein.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Alternative Name EGFR (EGFR Antibody Abstract)
Background Receptor for EGF, but also for other members of the EGF family, as TGF-a, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentia
Molecular Weight 175 kDa
NCBI Accession NP_005219
UniProt P00533
Research Area Cancer, Tyrosine Kinases, Growth Factors, Hormones, Receptors
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Background publications Moody, Scott, Amirghahari et al.: "Modulation of the cell growth regulator mTOR by Epstein-Barr virus-encoded LMP2A." in: Journal of virology, Vol. 79, Issue 9, pp. 5499-506, 2005 (PubMed).

Brugarolas, Lei, Hurley et al.: "Regulation of mTOR function in response to hypoxia by REDD1 and the TSC1/TSC2 tumor suppressor complex." in: Genes & development, Vol. 18, Issue 23, pp. 2893-904, 2004 (PubMed).

Kumar, Pandey, Sabatini et al.: "Functional interaction between RAFT1/FRAP/mTOR and protein kinase cdelta in the regulation of cap-dependent initiation of translation." in: The EMBO journal, Vol. 19, Issue 5, pp. 1087-97, 2000 (PubMed).

Gingras, Kennedy, OLeary et al.: "4E-BP1, a repressor of mRNA translation, is phosphorylated and inactivated by the Akt(PKB) signaling pathway." in: Genes & development, Vol. 12, Issue 4, pp. 502-13, 1998 (PubMed).

Burnett, Barrow, Cohen et al.: "RAFT1 phosphorylation of the translational regulators p70 S6 kinase and 4E-BP1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 4, pp. 1432-7, 1998 (PubMed).