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Insulin Receptor Substrate 1 (IRS1) (AA 637-641) antibody

Details for Product No. ABIN362887, Supplier: Log in to see
Antigen
Alternatives
anti-Human Insulin Receptor Substrate 1 antibody for Enzyme Immunoassay
Epitope
AA 637-641
55
49
44
44
24
23
21
21
16
16
15
14
13
13
13
13
13
13
9
9
8
7
6
5
5
4
4
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
514
354
337
11
9
5
1
1
Host
Rabbit
511
30
Clonality
Polyclonal
Conjugate
Un-conjugated
13
12
11
11
11
10
10
10
10
10
10
10
2
1
1
1
Application
Immunofluorescence (IF), Western Blotting (WB)
313
215
137
112
102
79
30
30
12
4
2
1
Supplier
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Immunogen Peptide sequence around AA 637-641 (P-K-S-V-S) derived from Human IRS-1. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
Isotype IgG
Specificity The antibody detects endogenous level of total IRS-1 protein.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific immunogen.
Alternative Name IRS-1 (IRS1 Antibody Abstract)
Background May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit
Molecular Weight 180 kDa
NCBI Accession NP_005535
UniProt P35568
Pathways Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes Western blotting: 1:500-1:1000
Immunofluorescence: 1:100-1:200
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Product cited in: Ekuni, Tomofuji, Irie et al.: "Effects of periodontitis on aortic insulin resistance in an obese rat model." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 90, Issue 3, pp. 348-59, 2010 (PubMed).

Background publications Tzatsos, Kandror: "Nutrients suppress phosphatidylinositol 3-kinase/Akt signaling via raptor-dependent mTOR-mediated insulin receptor substrate 1 phosphorylation." in: Molecular and cellular biology, Vol. 26, Issue 1, pp. 63-76, 2005 (PubMed).

Ozes, Akca, Mayo et al.: "A phosphatidylinositol 3-kinase/Akt/mTOR pathway mediates and PTEN antagonizes tumor necrosis factor inhibition of insulin signaling through insulin receptor substrate-1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 8, pp. 4640-5, 2001 (PubMed).

Kadowaki: "Insights into insulin resistance and type 2 diabetes from knockout mouse models." in: The Journal of clinical investigation, Vol. 106, Issue 4, pp. 459-65, 2000 (PubMed).

Szanto, Kahn: "Selective interaction between leptin and insulin signaling pathways in a hepatic cell line." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 5, pp. 2355-60, 2000 (PubMed).