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Cortactin antibody (CTTN) (AA 419-423)

Details for Product anti-CTTN Antibody No. ABIN362957, Supplier: Log in to see
Antigen
  • 1110020L01Rik
  • CG3637
  • cortactin
  • Cttn
  • CTTN
  • cttn
  • CTTN1
  • Cttnb
  • Dmel\CG3637
  • EMS1
  • Ems1
  • ems1
  • P85.25
Alternatives
anti-Mouse (Murine) Cortactin antibody for Western Blotting
Epitope
AA 419-423
28
26
17
15
14
12
9
7
6
6
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
180
106
60
8
7
5
3
2
2
1
1
1
1
1
1
Host
Rabbit
166
25
15
Clonality
Polyclonal
Conjugate
This Cortactin antibody is un-conjugated
4
4
4
4
4
4
Application
Immunohistochemistry (IHC)
171
89
85
22
16
9
7
3
3
2
1
1
1
Supplier
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Immunogen Peptide sequence around AA 419-423 (P-I-Y-E-D) derived from Human CORTACTIN. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
Isotype IgG
Specificity The antibody detects endogenous level of total Cortactin protein.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Alternative Name Cortactin (CTTN Antibody Abstract)
Background Cortactin is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Two splice variants that encode different isoforms have been identified for this gene.
Molecular Weight 85 kDa
NCBI Accession NP_005222
UniProt Q14247
Research Area Cancer, Cell Cycle, Cytoskeleton, Microfilaments
Pathways MAPK Signaling
Application Notes Immunohistochemistry: 1:50-1:100
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Background publications Luo, Pan, Mines, Watson, Zhang, Fan: "CXCL12 induces tyrosine phosphorylation of cortactin, which plays a role in CXC chemokine receptor 4-mediated extracellular signal-regulated kinase activation and chemotaxis." in: The Journal of biological chemistry, Vol. 281, Issue 40, pp. 30081-93, 2006

Head, Jiang, Li, Zorn, Schaefer, Parsons, Weed: "Cortactin tyrosine phosphorylation requires Rac1 activity and association with the cortical actin cytoskeleton." in: Molecular biology of the cell, Vol. 14, Issue 8, pp. 3216-29, 2003

Salomon, Ficarro, Brill, Brinker, Phung, Ericson, Sauer, Brock, Horn, Schultz, Peters: "Profiling of tyrosine phosphorylation pathways in human cells using mass spectrometry." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 2, pp. 443-8, 2003

Huang, Liu, Haudenschild, Zhan: "The role of tyrosine phosphorylation of cortactin in the locomotion of endothelial cells." in: The Journal of biological chemistry, Vol. 273, Issue 40, pp. 25770-6, 1998