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NFKBIE antibody (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, epsilon) (AA 20-24)

Details for Product anti-NFKBIE Antibody No. ABIN363012, Supplier: Log in to see
Antigen
  • IKBE
  • NFKBIE
  • Slc35b2
Alternatives
anti-Human NFKBIE antibody for Immunohistochemistry (Paraffin-embedded Sections)
Epitope
AA 20-24
35
13
13
4
3
2
2
2
2
2
2
2
2
2
1
1
Reactivity
Human, Mouse (Murine)
93
55
37
6
1
Host
Rabbit
94
2
Clonality
Polyclonal
Conjugate
This NFKBIE antibody is un-conjugated
4
4
3
3
3
3
3
3
3
3
3
3
Application
Immunohistochemistry (IHC), Western Blotting (WB)
60
36
33
18
12
10
3
2
Supplier
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Immunogen Peptide sequence around AA 20-24 (I-E-S-L-R) derived from Human IkB-e. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
Isotype IgG
Specificity The antibody detects endogenous level of total IkB-e protein.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Alternative Name IkB-epsilon (NFKBIE Antibody Abstract)
Background Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. Inhibits DNA-binding of NF-kappa-B p50-p65 and p50-c-Rel complexes.
Molecular Weight 40 kDa
NCBI Accession NP_004547
UniProt O00221
Pathways NF-kappaB Signaling
Application Notes Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Background publications McDevitt: "Discovering the role of the major histocompatibility complex in the immune response." in: Annual review of immunology, Vol. 18, pp. 1-17, 2000

Shirane, Hatakeyama, Hattori, Nakayama, Nakayama: "Common pathway for the ubiquitination of IkappaBalpha, IkappaBbeta, and IkappaBepsilon mediated by the F-box protein FWD1." in: The Journal of biological chemistry, Vol. 274, Issue 40, pp. 28169-74, 1999

Chen, Parent, Maniatis: "Site-specific phosphorylation of IkappaBalpha by a novel ubiquitination-dependent protein kinase activity." in: Cell, Vol. 84, Issue 6, pp. 853-62, 1996

Brown, Gerstberger, Carlson, Franzoso, Siebenlist: "Control of I kappa B-alpha proteolysis by site-specific, signal-induced phosphorylation." in: Science (New York, N.Y.), Vol. 267, Issue 5203, pp. 1485-8, 1995

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