Cofilin1/2 (CFL1/2) (AA 86-90) antibody

Details for Product No. ABIN363073
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Synonyms CFL
AA 86-90
(4), (1)
Human, Mouse (Murine)
(10), (7), (4)
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
(10), (5), (1)
Pubmed 4 references available
Quantity 50 μL
Shipping to United States ( )
Availability Will be delivered in 2 to 3 Business Days
Immunogen Peptide sequence around AA 86-90 (A-T-Y-E-T) derived from Human coflin1, cofilin2. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
Isotype IgG
Specificity The antibody detects endogenous level of total cofilin1/cofilin2 protein.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific immunogen.
Alternative Name cofilin1/cofilin2 (CFL1/2 Antibody Abstract)
Background Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.
Molecular Weight 19 kDa
NCBI Accession NP_005498, NP_068733
UniProt Q9Y281
Application Notes Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Product cited in: Li, Ke, Li et al.: "DGCR6L, a novel PAK4 interaction protein, regulates PAK4-mediated migration of human gastric cancer cell via LIMK1." in: The international journal of biochemistry & cell biology, Vol. 42, Issue 1, pp. 70-9, 2009 (PubMed).

Background publications Chen, Cizeau, Vande Velde et al.: "Nix and Nip3 form a subfamily of pro-apoptotic mitochondrial proteins." in: The Journal of biological chemistry, Vol. 274, Issue 1, pp. 7-10, 1999 (PubMed).

Yang, Higuchi, Ohashi et al.: "Cofilin phosphorylation by LIM-kinase 1 and its role in Rac-mediated actin reorganization." in: Nature, Vol. 393, Issue 6687, pp. 809-12, 1998 (PubMed).

Arber, Barbayannis, Hanser et al.: "Regulation of actin dynamics through phosphorylation of cofilin by LIM-kinase." in: Nature, Vol. 393, Issue 6687, pp. 805-9, 1998 (PubMed).

Catalog No. ABIN363073
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