Mouse anti-Human IgE (Fc Region) Antibody

Details for Product No. ABIN363708, Supplier: Log in to see
Antigen
Epitope
Fc Region
135
60
53
7
1
1
1
1
1
Reactivity
Human
489
141
46
44
6
6
3
2
2
2
1
1
1
Host
Mouse
296
286
67
52
10
6
3
1
1
Clonality (Clone)
Monoclonal ()
Application
Functional Studies (Func), Flow Cytometry (FACS), ELISA, Western Blotting (WB)
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Immunogen purified human myeloma IgE
Clone BSW17-NBS01
Isotype IgG1 kappa
Specificity Human IgE, Fce3-4, no cross-ractivity with other human or murine Ab isotypes
Characteristics IGHE, chromosome 14q32.33
Purification Affinity - purified from cell culture supernatant by Immunoaffinity Chromatography on CH-Sepharose 4B
Purity > 95 %, tested by analytical Gel Permeation Chromatography
Sterility Sterile filtered
Background The antibody recognizes a conformational epitope within the Ce3 domain of human IgE spanning towards the amino-terminal part of the Ce4 domain. It blocks the binding of IgE to its high affinity receptor on mast cells and basophils. It is therefore suited as an inhibitory reference compound for mast cell modulator profiling and basic research on IgE mediated mast cell receptor signaling.
Research Area Immunology, Secondary Antibodies
Application Notes For functional cellular assays (mast cell / basophil activation), when used at dilutions < 1:100, the sodium azide free formulation is recommended. Quality assurance: Reactivity > 99 %, compared to calibrator (Internal Standard mAb BSW17), tested by ELISA binding to immobilized human Standard IgE (SUS-11)
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer PBS, pH=7.2, sterile filtered, contains no NaN3
Handling Advice Monoclonal antibodies should not be stored at a temperature below -25 °C due to the aggregation effect of the protein.
Storage 4 °C
Supplier Images
 image for Mouse anti-Human IgE (Fc Region) antibody (ABIN363708) product purity: gel permeation chromatography (Superose 12/HR)
 image for Mouse anti-Human IgE (Fc Region) antibody (ABIN363708) Target binding kinetics (Biacore
Product cited in: Rudolf, Zuercher, Nechansky, Ruf, Vogel, Miescher, Stadler, Kricek: "Molecular basis for nonanaphylactogenicity of a monoclonal anti-IgE antibody." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 2, pp. 813-9, 2000 (PubMed).

Background publications Rudolf, Furukawa, Miescher, Vogel, Kricek, Stadler: "Effect of anti-IgE antibodies on Fc epsilonRI-bound IgE." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 12, pp. 5646-52, 1997 (PubMed).

Czech, Stadler, Schuopf, Kapp: "IgE autoantibodies in atopic dermatitis--occurrence of different antibodies against the CH3 and the CH4 epitopes of IgE." in: Allergy, Vol. 50, Issue 3, pp. 243-8, 1995 (PubMed).

Knutti-Mueller, Stadler, Magnusson, de Weck: "Human IgE synthesis in vitro. Detection with monoclonal antibodies." in: Allergy, Vol. 41, Issue 6, pp. 457-67, 1987 (PubMed).