TRIM3 antibody (AA 2-14)

Catalog No. ABIN364264

Product Details anti-TRIM3 Antibody

Target: TRIM3 (Tripartite Motif Containing 3 (TRIM3))

Binding Specificity: AA 2-14

Reactivity: Human, Mouse, Rat, Cow, Dog, Rabbit, Horse, Hamster, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This TRIM3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human TRIM3. Both variants (NP_006449.2, NP_150594.2,) represent the same protein. This antibody is expected to recognise all three human isoforms of this protein.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Horse, Rabbit (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide AKREDSPGPEVQP-C from the N-terminus of human TRIM3 (NP_006449.2, NP_150594.2). Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Horse, Rabbit (100%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:64000), WB (0.1 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for TRIM3

Target: TRIM3 (Tripartite Motif Containing 3 (TRIM3))

Alternative Name: TRIM3 (TRIM3 Products)

Synonyms: BERP antibody, HAC1 antibody, RNF22 antibody, RNF97 antibody, BERP1 antibody, Rnf22 antibody, Berp antibody, zgc:153967 antibody, tripartite motif containing 3 antibody, tripartite motif-containing 3 antibody, tripartite motif containing 3 S homeolog antibody, tripartite motif containing 3b antibody, TRIM3 antibody, Trim3 antibody, trim3.S antibody, trim3b antibody

Background: Name/Gene ID: TRIM3
Family: Tripartite Motif

Synonyms: TRIM3, BERP, Brain expressed ring finger, Tripartite motif containing 3, Tripartite motif protein TRIM3, RING finger protein 97, RNF22, RNF97, HAC1, RING finger protein 22, Tripartite motif-containing 3

Gene ID: 10612

NCBI Accession: NP_006449, NP_150594

UniProt: O75382