TRIM3 antibody (AA 2-14)
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- Target See all TRIM3 Antibodies
- TRIM3 (Tripartite Motif Containing 3 (TRIM3))
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Binding Specificity
- AA 2-14
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Reactivity
- Human, Mouse, Rat, Cow, Dog, Rabbit, Horse, Hamster, Monkey
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This TRIM3 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Brand
- IHC-plus™
- Specificity
- Human TRIM3. Both variants (NP_006449.2, NP_150594.2,) represent the same protein. This antibody is expected to recognise all three human isoforms of this protein.
- Predicted Reactivity
- Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Horse, Rabbit (100%).
- Purification
- Immunoaffinity purified
- Immunogen
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Synthetic peptide AKREDSPGPEVQP-C from the N-terminus of human TRIM3 (NP_006449.2, NP_150594.2). Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Horse, Rabbit (100%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product TRIM3 Primary Antibody
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- Application Notes
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Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:64000), WB (0.1 - 1 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Tris-buffered saline, pH 7.3, 0.5 % BSA, 0.02 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Store at -20°C. Avoid freeze-thaw cycles.
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- Target
- TRIM3 (Tripartite Motif Containing 3 (TRIM3))
- Alternative Name
- TRIM3 (TRIM3 Products)
- Synonyms
- BERP antibody, HAC1 antibody, RNF22 antibody, RNF97 antibody, BERP1 antibody, Rnf22 antibody, Berp antibody, zgc:153967 antibody, tripartite motif containing 3 antibody, tripartite motif-containing 3 antibody, tripartite motif containing 3 S homeolog antibody, tripartite motif containing 3b antibody, TRIM3 antibody, Trim3 antibody, trim3.S antibody, trim3b antibody
- Background
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Name/Gene ID: TRIM3
Family: Tripartite Motif
Synonyms: TRIM3, BERP, Brain expressed ring finger, Tripartite motif containing 3, Tripartite motif protein TRIM3, RING finger protein 97, RNF22, RNF97, HAC1, RING finger protein 22, Tripartite motif-containing 3 - Gene ID
- 10612
- NCBI Accession
- NP_006449, NP_150594
- UniProt
- O75382
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