ENO2/NSE antibody

Catalog No. ABIN364284

The Mouse Monoclonal anti-ENO2/NSE antibody (Clone NSE-P1) (ABIN364284) specifically detects ENO2/NSE in WB, IHC, ELISA and IHC (p). The antibody is reactive with Human samples.

Quick Overview for ENO2/NSE antibody (ABIN364284)

Target: ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ENO2/NSE antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: NSE-P1

Product Details anti-ENO2/NSE Antibody

Specificity: Anti neuron-specific enolase 1 (NSE-P1, ENO1, Enolase 1, 2-Phospho-D-Glycerate Hydrolase, alpha enolase, ay) is an 80 kD protein and one of the five isozymes of enolase, a glycolytic enzyme catalyzing the reaction pathway between 2 phospho glycerate and p.

Purification: Protein G purified

Immunogen: Enolase of human origin.

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (20 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 20 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C (no longer than 1 week)
Long term -20°C
Aliquot to avoid freeze/thaw cycles.

Target Details for ENO2/NSE

Target: ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))

Alternative Name: ENO2 / NSE

Background: Name/Gene ID: ENO2

Synonyms: ENO2, Enolase 2, Enolase 2 (gamma, neuronal), Neurone-specific enolase, Gamma-enolase, Neural enolase, Neuron specific gamma enolase, Neuron-specific enolase, NSE

Gene ID: 2026

UniProt: P09104