Syndecan 2 antibody (Internal Region)

Catalog No. ABIN364375

Product Details anti-Syndecan 2 Antibody

Target: Syndecan 2 (SDC2)

Binding Specificity: Internal Region

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Syndecan 2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Recognizes human syndecan-2 (fibroglycan). It recognizes the unglyconated form and may recognize the glyconated form. On Western Blots, it identifies a single band at ~22kD. An additional band at ~40kD may also be observed which may represent a glycanated form. Reactivity has been confirmed with human A-549 lung carcinoma, HEP-G2 hepatocellular carcinoma, HT-29 colorectal adenocarcinoma, K-562 chronic myelogenous leukemia-bone marrow, TF-1 erythroleukemia-bone marrow and U-2 OS osteosarcoma cell lysates.

Purification: Affinity purified

Immunogen: Synthetic peptide derived from the internal region of human syndecan-2 (fibroglycan).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (0.1 - 1 μg/mL), IHC, IHC-P (5 μg/mL), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Syndecan 2

Target: Syndecan 2 (SDC2)

Alternative Name: SDC2 / Syndecan 2 (SDC2 Products)

Synonyms: CD362 antibody, HSPG antibody, HSPG1 antibody, SYND2 antibody, 4833414L08Rik antibody, AA960457 antibody, Hspg1 antibody, Synd2 antibody, syndecan-2 antibody, cb440 antibody, zgc:103676 antibody, sdc2 antibody, SYND2-B antibody, Xsyn-2 antibody, hspg antibody, hspg1 antibody, sdc2-a antibody, sdc2-b antibody, synd2 antibody, syndecan 2 antibody, syndecan 2 L homeolog antibody, syndecan 2 S homeolog antibody, SDC2 antibody, Sdc2 antibody, sdc2 antibody, sdc2.L antibody, sdc2.S antibody

Background: Name/Gene ID: SDC2

Synonyms: SDC2, CD362, CD362 antigen, HSPG, HSPG1, Fibroglycan, Syndecan proteoglycan 2, SYND2, Syndecan 2, Syndecan-2

Gene ID: 6383

UniProt: P34741

Pathways: Glycosaminoglycan Metabolic Process