CENPF antibody (AA 3198-3210)
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- Target See all CENPF Antibodies
- CENPF (Centromere Protein F (CENPF))
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Binding Specificity
- AA 3198-3210
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Reactivity
- Human
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This CENPF antibody is un-conjugated
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Application
- ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Brand
- IHC-plus™
- Specificity
- Human CENPF. The immunizing peptide shows one mismatch with the corresponmding sequence of the NCBI-curated isoform NP_057427.3.
- Predicted Reactivity
- Percent identity by BLAST analysis: Gorilla (100%) Human, Monkey (92%) Gibbon, Bovine (85%).
- Purification
- Immunoaffinity purified
- Immunogen
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Synthetic peptide GLESKGSENCKVQ from the C-terminus of human CENPF (AAA82889.1, AAA82935.1, AAA86889.1). Percent identity by BLAST analysis: Gorilla (100%), Human, Monkey (92%), Gibbon, Bovine (85%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product CENPF Primary Antibody
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- Application Notes
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Approved: IHC, IHC-P (3.75 μg/mL), Peptide-ELISA (1:64000)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 3.75 μg/mL. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Tris-buffered saline, pH 7.3, 0.5 % BSA, 0.02 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Store at -20°C. Avoid freeze-thaw cycles.
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- Target
- CENPF (Centromere Protein F (CENPF))
- Alternative Name
- CENPF / CENP-F (CENPF Products)
- Synonyms
- LOC100222985 antibody, 6530404A22Rik antibody, AI325968 antibody, CENF antibody, Lek1 antibody, PRO1779 antibody, mitosin antibody, im:7140452 antibody, im:7144238 antibody, im:7154719 antibody, im:7156761 antibody, si:ch211-152h14.2 antibody, hcp-1 antibody, CMF1 antibody, centromere protein F antibody, CENPF antibody, Cenpf antibody, cenpf antibody
- Background
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Name/Gene ID: CENPF
Synonyms: CENPF, AH antigen, Centromere protein F, CENF, CENP-F, Kinetochore protein CENPF, Mitosin, CENP-F kinetochore protein, Hcp-1, PRO1779 - Gene ID
- 1063
- UniProt
- P49454
- Pathways
- Chromatin Binding, M Phase, SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection
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