RNF34 antibody (AA 3-14)

Catalog No. ABIN364389

Product Details anti-RNF34 Antibody

Target: RNF34 (Ring Finger Protein 34 (RNF34))

Binding Specificity: AA 3-14

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Horse, Hamster

Host: Goat

Clonality: Polyclonal

Conjugate: This RNF34 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human RNF34. This antibody is expected to recognize both reported isoforms (NP_919247.1 and NP_079402.2).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Marmoset, Mouse, Rat, Hamster, Bovine, Horse, Rabbit (100%) Monkey, Elephant, Panda, Dog, Bat, Opossum, Turkey, Platypus, Xenopus, Salmon, Stickleback, Zebrafish (92%) Pufferfish (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide KAGATSMWASCC from the N-terminus of human RNF34 (NP_919247.1, NP_079402.2). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Marmoset, Mouse, Rat, Hamster, Bovine, Horse, Rabbit (100%), Monkey, Elephant, Panda, Dog, Bat, Opossum, Turkey, Platypus, Xenopus, Salmon, Stickleback, Zebrafish (92%), Pufferfish (83%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (3.75 μg/mL), Peptide-ELISA (1:32000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 3.75 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for RNF34

Target: RNF34 (Ring Finger Protein 34 (RNF34))

Alternative Name: RNF34 (RNF34 Products)

Synonyms: CARP-1 antibody, CARP1 antibody, RFI antibody, RIF antibody, RIFF antibody, hRFI antibody, rnf34 antibody, AW061037 antibody, AW536122 antibody, BC004042 antibody, C88279 antibody, Momo antibody, fi35g06 antibody, wu:fi35g06 antibody, zgc:114043 antibody, zgc:171755 antibody, ring finger protein 34 antibody, E3 ubiquitin-protein ligase RNF34 antibody, ring finger protein 34a antibody, ring finger protein 34b antibody, Residual feed intake antibody, RNF34 antibody, rnf34 antibody, Rnf34 antibody, rnf34a antibody, rnf34b antibody, RFI antibody

Background: Name/Gene ID: RNF34

Synonyms: RNF34, CARP1, Caspase regulator CARP1, CARP-1, FYVE-RING finger protein Momo, HRFI, RFI, RIF, RIFF, RING finger protein 34, RING finger protein RIFF

Gene ID: 80196

NCBI Accession: NP_919247, NP_079402

UniProt: Q969K3