FTH1 antibody (AA 221-232)

Catalog No. ABIN364417

Product Details anti-FTH1 Antibody

Target: FTH1 (Ferritin, Heavy Polypeptide 1 (FTH1))

Binding Specificity: AA 221-232

Reactivity: Human, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This FTH1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human FTH1.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Opossum, Platypus (100%) Dolphin, Dog, Rabbit (92%) Panda, Cat, Armadillo (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-DKHTLGDSDNES from the C-terminus of human FTH1 (NP_002023.2). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Opossum, Platypus (100%), Dolphin, Dog, Rabbit (92%), Panda, Cat, Armadillo (83%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (4 μg/mL), Peptide-ELISA (1:8000), WB (0.03 - 0.1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 4 μg/mL. Peptide ELISA: antibody detection limit dilution 1:8000. Western Blot: Approx. 22kD band observed in Human Brain (Cerebellum), Liver and Placenta lysates (calculated MW of 21.2kD according to NP_002023.2). Recommended concentration: 0.03-0.1 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for FTH1

Target: FTH1 (Ferritin, Heavy Polypeptide 1 (FTH1))

Alternative Name: Ferritin / FTH1 (FTH1 Products)

Synonyms: FHC antibody, FTH antibody, FTHL6 antibody, PIG15 antibody, PLIF antibody, apoferritin antibody, ferritin antibody, fhc antibody, fth antibody, fth1 antibody, fthl6 antibody, ftn-2 antibody, pig15 antibody, plif antibody, fth1b antibody, Fth antibody, HFt antibody, MFH antibody, fb06g09 antibody, hm:zeh1145 antibody, wu:fb06g09 antibody, wu:fq18c10 antibody, zeh1145 antibody, ferritin heavy chain 1 antibody, ferritin, heavy polypeptide 1 L homeolog antibody, ferritin, heavy polypeptide 1 S homeolog antibody, ferritin heavy polypeptide 1 antibody, ferritin, heavy polypeptide 1a antibody, ferritin antibody, ferritin, heavy polypeptide 1 antibody, tudor domain containing 9 antibody, ferritin, heavy polypeptide 1 a antibody, FTH1 antibody, fth1.L antibody, fth1.S antibody, Fth1 antibody, fth1a antibody, EAMY_RS19690 antibody, fth1 antibody, TDRD9 antibody

Background: Name/Gene ID: FTH1

Synonyms: FTH1, Apoferritin, Ferritin H subunit, Ferritin heavy chain, Ferritin, heavy polypeptide 1, FHC, FTHL6, PIG15, PLIF, FTH

Gene ID: 2495

NCBI Accession: NP_002023

UniProt: P02794

Pathways: Transition Metal Ion Homeostasis