CARD11 antibody (AA 1160-1171)

Catalog No. ABIN364421

Product Details anti-CARD11 Antibody

Target: CARD11 (Caspase Recruitment Domain Family, Member 11 (CARD11))

Binding Specificity: AA 1160-1171

Reactivity: Human, Mouse, Rat, Hamster, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This CARD11 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human CARD11.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Rat, Hamster (100%) Elephant, Panda, Bovine, Dog, Horse, Turkey, Chicken (92%) Opossum (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-QRKTIWVDEDQL from the C-terminus of human CARD11 (NP_115791.3). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Rat, Hamster (100%), Elephant, Panda, Bovine, Dog, Horse, Turkey, Chicken (92%), Opossum (83%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2 μg/mL), Peptide-ELISA (1:32000), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for CARD11

Target: CARD11 (Caspase Recruitment Domain Family, Member 11 (CARD11))

Alternative Name: CARMA1 / CARD11 (CARD11 Products)

Synonyms: BENTA antibody, BIMP3 antibody, CARMA1 antibody, PPBL antibody, 0610008L17Rik antibody, 2410011D02Rik antibody, CARD11 antibody, LOC100232755 antibody, caspase recruitment domain family member 11 antibody, caspase recruitment domain family, member 11 antibody, caspase recruitment domain-containing protein 11 antibody, CARD11 antibody, Card11 antibody, LOC100550812 antibody, card11 antibody

Background: Name/Gene ID: CARD11
Family: Apoptosis

Synonyms: CARD11, Card-maguk protein 1, CARMA1, Carma 1, BIMP3

Gene ID: 84433

NCBI Accession: NP_115791

UniProt: Q9BXL7

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, BCR Signaling