PLIN1 antibody (AA 488-499)

Catalog No. ABIN364425

Product Details anti-PLIN1 Antibody

Target: PLIN1 (Perilipin 1 (PLIN1))

Binding Specificity: AA 488-499

Reactivity: Human, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This PLIN1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human PLIN1 / Perilipin.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gibbon, Monkey, Marmoset (100%) Mouse, Rat, Hamster, Elephant, Bovine, Pig (92%) Gorilla, Panda, Bat, Horse, Opossum (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-PREKPKRRVSDS from an internal region (near the C-terminus) of human PLIN1 / Perilipin (NP_002657.2). Percent identity by BLAST analysis: Human, Gibbon, Monkey, Marmoset (100%), Mouse, Rat, Hamster, Elephant, Bovine, Pig (92%), Gorilla, Panda, Bat, Horse, Opossum (83%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2 μg/mL), Peptide-ELISA (1:128000), WB (0.01 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2 μg/mL.

Not recommended for: IF

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for PLIN1

Target: PLIN1 (Perilipin 1 (PLIN1))

Alternative Name: PLIN1 / Perilipin (PLIN1 Products)

Synonyms: FPLD4 antibody, PERI antibody, PLIN antibody, PERIA antibody, Plin antibody, 6030432J05Rik antibody, Peri antibody, peri antibody, plin antibody, perilipin antibody, LOC692833 antibody, perilipin 1 antibody, perilipin antibody, perilipin 1 L homeolog antibody, PLIN1 antibody, Plin1 antibody, plin1 antibody, LOC692833 antibody, plin1.L antibody

Background: Name/Gene ID: PLIN1

Synonyms: PLIN1, FPLD4, PERI, Perilipin-1, PLIN, Perilipin, Perilipin 1

Gene ID: 5346

NCBI Accession: NP_002657

UniProt: O60240

Pathways: Lipid Metabolism