Papillomavirus (HPV) antibody

Antigen: Papillomavirus (HPV)

Clonality: Monoclonal (K1H8)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Certificates: ISO 9001:2008, ISO 13485:2003

Supplier: Dako

Catalog no.: ABIN370638

Additional Information

Immunogen: Alkaline disrupted HPV type 1 (2)

Isotype: IgG1, kappa chain  (Matching secondary antibodies)

Clone: K1H8

Description: HPV is a non-enveloped DNA virus comprised of an icosahedral capsid and circular, double-stranded DNA. HPV is classified into more than 50 distinct types based on base sequences (1).

Specificity: Anti-human papillomavirus, clone K1H8 (anti-HPV) reacts with a non-conformational internal linear epitope of a major capsid protein of HPV-1, which is broadly expressed among the different HPV subtypes. In Western blotting, anti-HPV identified a 57 kD protein, which corresponds to the molecular weight of a major polypeptide of the capsid protein VP1. Minor bands of 47, 44, 41 and 33 kD were weakly labelled and thought to be degraded capsid proteins. Anti-HPV has been demonstrated to identify HPV-1 antigen in ELISA and to detect HPV-1 and HPV-6 by indirect immunofluorescence in infected frozen tissue sections. Anti-HPV was found to be immunoreactive with paraffin sections of formalin-fixed HPV-infected tissues which were demonstrated by Southern blot hybridization to be infected with HPV type 6, 11, 16, 18, 31, 33, 42, 51, 52, 56 and 58. Positive immunostaining was largely confined to the nuclei of infected cells. Occasionally, the cytoplasm of koilocytotic cells was observed to be immunoreactive (2).

Application Details

Application Notes: Paraffin Sections Anti-endoglin, SN6h, can be used on formalin-fixed, paraffin-embedded tissue sections. In order for this antibody to perform optimally on paraffin-embedded tissues, a high sensitivity detection system is required, such as CSA (code K1500) or LSAB+ HRP (code K0679). Monoclonal anti-endoglin, SN6h, may be used at a dilution of 1:2000 in the CSA detection system, determined on formalin-fixed, paraffin-embedded tissue. When using the CSA detection system, pretreatment of tissue with proteolytic enzymes is not recommended. Anti-endoglin, SN6h, may also be used at a dilution of 1:5 to 1:10 with other high sensitivity detection systems such as the LSAB+ HRP method. Pre-treatment of formalin-fixed, paraffin-embedded tissue sections with proteolytic enzymes should be performed prior to staining. When using anti-endoglin with this detection system, one may observe a relative decrease in sensitivity compared to results obtained with the CSA system. These recommended dilutions are guidelines only, optimal dilutions should be determined by the individual laboratory. (110937-001) 303333EFG_001 p. 2/5 Cryostat Sections and Cell Smears Anti-endoglin can also be used to label cryostat sections or cell smears with the LSAB method. Staining interpretation Follow the recommended procedure for the detection system selected. Specimen preparation The cellular staining pattern for anti-endoglin is cytoplasmic. Normal Cells Endoglin has been found on endothelial cells of capillaries, arterioles, small arteries, venules and high endothelial venules in a variety of tissues. Positive tissues include liver, kidney, skin, urethra, thyroid, lymph node, tonsil, umbilical cord, ovarian tube, thymus, spleen, and lung. In tonsil, lymph node and thymus, all endothelial cells, including those of high endothelial venules, express endoglin. 4,8,9 Endoglin expression has also been demonstrated on immature proerythroblasts, syncytiotrophoblasts, activated macrophages and stromal fibroblasts from adult and fetal lung and skin. 4,9,10 Tumor Cells Endoglin expression has been demonstrated on non-T/non-B and pre-B acute lymphoblastic leukemia (ALL) and acute myelocytic and myelo-monocytic leukemia cells. 6,8 Hairy Cell leukemic cells have also been found to express endoglin.

Buffer: Monoclonal Mouse antibody provided in liquid form as tissue culture supernatant in 0.05 mol/L Tris-HCl, pH 7.2 and 0.015 mol/L sodium azide. This product contains stabilizing protein.

Storage: Store at 2-8 °C. Precautions: 1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. For professional users. 3. This product contains sodium azide (NaN 3 ), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, NaN 3 may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 4. As with any product derived from biological sources, proper handling procedures should be used . 5. Wear appropriate Personal Protective Equipment to avoid contact with eyes and skin. 6. Unused reagents should be disposed of according to local, State, and Federal regulations.

Restrictions: For Research Use only

Publications

Publications: Xu, Stolk, Zhang et al.: "Identification of differentially expressed genes in human prostate cancer using subtraction and microarray." in: Cancer research, Vol. 60, Issue 6, pp. 1677-82, 2000 (PubMed).

Ferdinandusse, Denis, IJlst et al.: "Subcellular localization and physiological role of alpha-methylacyl-CoA racemase." in: Journal of lipid research, Vol. 41, Issue 11, pp. 1890-6, 2000 (PubMed).

Jiang, Woda, Rock et al.: "P504S: a new molecular marker for the detection of prostate carcinoma." in: The American journal of surgical pathology, Vol. 25, Issue 11, pp. 1397-404, 2001 (PubMed).

Yang, Wu, Woda et al.: "Expression of alpha-Methylacyl-CoA racemase (P504S) in atypical adenomatous hyperplasia of the prostate." in: The American journal of surgical pathology, Vol. 26, Issue 7, pp. 921-5, 2002 (PubMed).

Jiang, Wu, Woda et al.: "P504S/alpha-methylacyl-CoA racemase: a useful marker for diagnosis of small foci of prostatic carcinoma on needle biopsy." in: The American journal of surgical pathology, Vol. 26, Issue 9, pp. 1169-74, 2002 (PubMed).

Beach, Gown, De Peralta-Venturina et al.: "P504S immunohistochemical detection in 405 prostatic specimens including 376 18-gauge needle biopsies." in: The American journal of surgical pathology, Vol. 26, Issue 12, pp. 1588-96, 2002 (PubMed).

Zhou, Jiang, Epstein: "Expression and diagnostic utility of alpha-methylacyl-CoA-racemase (P504S) in foamy gland and pseudohyperplastic prostate cancer." in: The American journal of surgical pathology, Vol. 27, Issue 6, pp. 772-8, 2003 (PubMed).

Jiang, Fanger, Woda et al.: "Expression of alpha-methylacyl-CoA racemase (P504s) in various malignant neoplasms and normal tissues: astudy of 761 cases." in: Human pathology, Vol. 34, Issue 8, pp. 792-6, 2003 (PubMed).

Jiang, Fanger, Banner et al.: "A dietary enzyme: alpha-methylacyl-CoA racemase/P504S is overexpressed in colon carcinoma." in: Cancer detection and prevention, Vol. 27, Issue 6, pp. 422-6, 2003 (PubMed).

Tretiakova, Sahoo, Takahashi et al.: "Expression of alpha-methylacyl-CoA racemase in papillary renal cell carcinoma." in: The American journal of surgical pathology, Vol. 28, Issue 1, pp. 69-76, 2004 (PubMed).

Wu, Yang, Tretiakova et al.: "Analysis of alpha-methylacyl-CoA racemase (P504S) expression in high-grade prostatic intraepithelial neoplasia." in: Human pathology, Vol. 35, Issue 8, pp. 1008-13, 2004 (PubMed).

Jiang, Woda, Wu et al.: "Discovery and clinical application of a novel prostate cancer marker: alpha-methylacyl CoA racemase (P504S)." in: American journal of clinical pathology, Vol. 122, Issue 2, pp. 275-89, 2004 (PubMed).

Logani, Oliva, Arnell et al.: "Use of novel immunohistochemical markers expressed in colonic adenocarcinoma to distinguish primary ovarian tumors from metastatic colorectal carcinoma." in: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, Vol. 18, Issue 1, pp. 19-25, 2005 (PubMed).

Witkiewicz, Varambally, Shen et al.: "Alpha-methylacyl-CoA racemase protein expression is associated with the degree of differentiation in breast cancer using quantitative image analysis." in: Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, Vol. 14, Issue 6, pp. 1418-23, 2005 (PubMed).

Nassar, Amin, Sexton et al.: "Utility of alpha-methylacyl coenzyme A racemase (p504s antibody) as a diagnostic immunohistochemical marker for cancer." in: Applied immunohistochemistry & molecular morphology : AIMM / official publication of the Society for Applied Immunohistochemistry, Vol. 13, Issue 3, pp. 252-5, 2005 (PubMed).

Moliniue, Balaton, Rotman et al.: "Alpha-methyl CoA racemase expression in renal cell carcinomas." in: Human pathology, Vol. 37, Issue 6, pp. 698-703, 2006 (PubMed).

Cho, Kim, Park et al.: "AMACR is highly expressed in gastric adenomas and intestinal-type carcinomas." in: APMIS : acta pathologica, microbiologica, et immunologica Scandinavica, Vol. 115, Issue 6, pp. 713-8, 2007 (PubMed).

Lee: "Alpha-methylacyl-CoA-racemase expression in adenocarcinoma, dysplasia and non-neoplastic epithelium of the stomach." in: Oncology, Vol. 71, Issue 3-4, pp. 246-50, 2007 (PubMed).

Osunkoya, Netto, Epstein: "Colorectal adenocarcinoma involving the prostate: report of 9 cases." in: Human pathology, Vol. 38, Issue 12, pp. 1836-41, 2007 (PubMed).

Shilo, Dracheva, Mani et al.: "Alpha-methylacyl CoA racemase in pulmonary adenocarcinoma, squamous cell carcinoma, and neuroendocrine tumors: expression and survival analysis." in: Archives of pathology & laboratory medicine, Vol. 131, Issue 10, pp. 1555-60, 2007 (PubMed).

Li, Cagle, Botero et al.: "Significance of overexpression of alpha methylacyl-coenzyme A racemase in hepatocellular carcinoma." in: Journal of experimental & clinical cancer research : CR, Vol. 27, pp. 2, 2008 (PubMed).

Truong, Li, Feng et al.: "Alpha-methylacyl-CoA racemase expression is upregulated in gastric adenocarcinoma: a study of 249 cases." in: International journal of clinical and experimental pathology, Vol. 1, Issue 6, pp. 518-23, 2008 (PubMed).

Sun, Huan, Unger: "Clear cell adenocarcinoma of urinary bladder and urethra: another urinary tract lesion immunoreactive for P504S." in: Archives of pathology & laboratory medicine, Vol. 132, Issue 9, pp. 1417-22, 2008 (PubMed).