CD11b antibody (Integrin alpha M)

Details for Product anti-ITGAM Antibody No. ABIN370849, Supplier: Log in to see
Antigen
  • CD11b/CD18
  • CR3
  • CR3A
  • Cd11b
  • F730045J24Rik
  • Ly-40
  • MAC1
  • Mac-1
  • Mac-1a
  • CD11B
  • MAC-1
  • MAC1A
  • MO1A
  • SLEB6
  • C3bi
  • integrin, alpha M (complement component 3 receptor 3 subunit)
  • integrin alpha M
  • integrin, alpha M
  • ITGAM
  • Itgam
Alternatives
anti-Human CD11b antibody for Immunoprecipitation
Reactivity
Mouse (Murine)
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Host
Rat
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2
Clonality (Clone)
Monoclonal ()
Conjugate
This CD11b antibody is un-conjugated
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124
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Application
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
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Options
Supplier
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Immunogen Peritoneal macrophages from C57 B1/6 x DBA/2 F1 hybrid mice. (Ref. 1)
Clone 3A33
Isotype IgG2a
Characteristics Synonyms: Integrin alpha-M, CR3A, MAC-1, MAC1, CR-3 alpha chain, Cell surface glycoprotein MAC-1subunit alpha, Leukocyte adhesion receptor MO1, Neutrophil adherence receptor, CD11antigen-like family member B
Endotoxin Level Low Endotoxin
Alternative Name CD11b (ITGAM Antibody Abstract)
Background CD11b (Integrin alpha M subunit) combines with CD18 (Integrin beta2 subunit) to form the integrin Mac-1, also known as complement receptor 3 (CR3), which mediates adhesion to C3bi and ICAM-1 (CD54). (Ref.2-6) Mac-1 is expressed at varying levels on granulocytes, macrophages, dendritic cells, NK cells, and B-1 cells in the peritoneal and pleural cavities. (Ref.2,7-10) In addition to its role in binding C3bi on opsonized targets and mediation of the subsequent ingestion process. (Ref.4,11) Mac-1 is important as an adhesion molecule in the transendothelial migration of monocytes and neutrophils. (Ref.12)Synonyms: CD11 antigen-like family member B, CR-3 alpha chain, CR3A, Cell surface glycoprotein MAC-1 subunit alpha, ITGAM, Integrin alpha-M, Leukocyte adhesion receptor MO1, MAC1, Neutrophil adherence receptor
UniProt P05555
Research Area Stem Cells, Hematopoietic Progenitors, Hematopoietic Stem Cells, Glia marker, CD Antigens, Surface Receptors of Immune Cells
Pathways Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation
Application Notes Flow Cytometry Immunohistochemistry on Frozen Sections. (Ref.13)Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer PBS without preservatives.
Preservative Without preservative
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months
Background publications Lub, van Kooyk, Figdor: "Competition between lymphocyte function-associated antigen 1 (CD11a/CD18) and Mac-1 (CD11b/CD18) for binding to intercellular adhesion molecule-1 (CD54)." in: Journal of leukocyte biology, Vol. 59, Issue 5, pp. 648-55, 1996 (PubMed).

Leenen, de Bruijn, Voerman, Campbell, van Ewijk: "Markers of mouse macrophage development detected by monoclonal antibodies." in: Journal of immunological methods, Vol. 174, Issue 1-2, pp. 5-19, 1994 (PubMed).

Vremec, Zorbas, Scollay, Saunders, Ardavin, Wu, Shortman: "The surface phenotype of dendritic cells purified from mouse thymus and spleen: investigation of the CD8 expression by a subpopulation of dendritic cells." in: The Journal of experimental medicine, Vol. 176, Issue 1, pp. 47-58, 1992 (PubMed).

Kantor, Stall, Adams, Herzenberg, Herzenberg: "Differential development of progenitor activity for three B-cell lineages." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 8, pp. 3320-4, 1992 (PubMed).

Gresham, Graham, Anderson, Brown: "Leukocyte adhesion-deficient neutrophils fail to amplify phagocytic function in response to stimulation. Evidence for CD11b/CD18-dependent and -independent mechanisms of phagocytosis." in: The Journal of clinical investigation, Vol. 88, Issue 2, pp. 588-97, 1991 (PubMed).

Martin, Le Corre, Pellen, Bourel, Merdrignac, Genetet, Toujas: "A monoclonal antibody (3A33) that reacts with a mouse-specific epitope of Mac-1 antigen." in: Tissue antigens, Vol. 28, Issue 1, pp. 15-23, 1986 (PubMed).

Springer, Davignon, Ho, Kürzinger, Martz, Sanchez-Madrid: "LFA-1 and Lyt-2,3, molecules associated with T lymphocyte-mediated killing; and Mac-1, an LFA-1 homologue associated with complement receptor function." in: Immunological reviews, Vol. 68, pp. 171-95, 1983 (PubMed).

Beller, Springer, Schreiber: "Anti-Mac-1 selectively inhibits the mouse and human type three complement receptor." in: The Journal of experimental medicine, Vol. 156, Issue 4, pp. 1000-9, 1983 (PubMed).

Sanchez-Madrid, Simon, Thompson, Springer: "Mapping of antigenic and functional epitopes on the alpha- and beta-subunits of two related mouse glycoproteins involved in cell interactions, LFA-1 and Mac-1." in: The Journal of experimental medicine, Vol. 158, Issue 2, pp. 586-602, 1983 (PubMed).

Ault, Springer: "Cross-reaction of a rat-anti-mouse phagocyte-specific monoclonal antibody (anti-Mac-1) with human monocytes and natural killer cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 126, Issue 1, pp. 359-64, 1981 (PubMed).

Springer, Galfré, Secher, Milstein: "Mac-1: a macrophage differentiation antigen identified by monoclonal antibody." in: European journal of immunology, Vol. 9, Issue 4, pp. 301-6, 1979 (PubMed).

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