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CD40 Ligand antibody (CD40LG)

Details for Product anti-CD40LG Antibody No. ABIN370947, Supplier: Log in to see
Antigen
  • CD40-L
  • Cd40l
  • CD40L
  • cd40l
  • CD154
  • gp39
  • hCD40L
  • HIGM1
  • IGM
  • IMD3
  • Ly-62
  • Ly62
  • T-BAM
  • Tnfsf5
  • TNFSF5
  • TRAP
Alternatives
anti-Mouse (Murine) CD40 Ligand antibody for Flow Cytometry
Reactivity
Mouse (Murine)
456
211
40
15
14
11
7
4
3
3
3
2
2
2
2
Host
Hamster
286
146
102
57
28
26
Clonality (Clone)
Monoclonal ()
Conjugate
This CD40 Ligand antibody is un-conjugated
80
61
43
11
11
9
9
8
8
8
7
7
7
7
7
7
7
6
6
6
4
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Functional Studies (Func), Flow Cytometry (FACS), Immunoprecipitation (IP)
379
250
171
59
58
54
52
50
41
22
16
16
11
7
6
6
5
4
3
3
3
2
2
1
1
1
1
1
Supplier
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Immunogen Activated mouse Th1 clone D1. 6 (Ref. 1)
Clone MR1
Isotype IgG
Characteristics Synonyms: CD40 ligand, CD40-L, CD40LG, TNFSF5, TRAP, GP39, Tumor necrosis factor ligandsuperfamily member 5, TNF-related activation protein, T-cell antigen Gp39
Endotoxin Level Low Endotoxin
Alternative Name CD154 / CD40L (CD40LG Antibody Abstract)
Background CD154, formerly known as CD40 ligand and gp39, is a type II integral membrane protein and a member of the tumor necrosis factor (TNF) family of ligands. (Ref.1-7) It is an important accessory molecule in T cell-B cell costimulatory interactions, and is expressed predominantly on activated CD4+ T lymphocytes. It is also present on the surface of activated Th0, Th1, and Th2 T cell clones. Its expression is transient and cyclosporinsensitive. (Ref.6)Synonyms: CD40 ligand, CD40-L, CD40LG, GP39, T-cell antigen Gp39, TNF-related activation protein, TNFSF5, TRAP, Tumor necrosis factor ligand superfamily member 5
UniProt P27548
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways NF-kappaB Signaling
Application Notes Flow Cytometry. (Ref.1,5)Immunoprecipitation. Functional Assay: In vivo and in vitro blockage of CD154 function. (Ref.1,3,5)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer PBS containing no preservatives.
Preservative Without preservative
Storage -20 °C
Storage Comment Store the antibody undiluted (in aliquots) at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months
Background publications Foy, Page, Waldschmidt, Schoneveld, Laman, Masters, Tygrett, Ledbetter, Aruffo, Claassen, Xu, Flavell, Oehen, Hedrick, Noelle: "An essential role for gp39, the ligand for CD40, in thymic selection." in: The Journal of experimental medicine, Vol. 182, Issue 5, pp. 1377-88, 1995 (PubMed).

Aruffo, Farrington, Hollenbaugh, Li, Milatovich, Nonoyama, Bajorath, Grosmaire, Stenkamp, Neubauer: "The CD40 ligand, gp39, is defective in activated T cells from patients with X-linked hyper-IgM syndrome." in: Cell, Vol. 72, Issue 2, pp. 291-300, 1993 (PubMed).

Foy, Shepherd, Durie, Aruffo, Ledbetter, Noelle: "In vivo CD40-gp39 interactions are essential for thymus-dependent humoral immunity. II. Prolonged suppression of the humoral immune response by an antibody to the ligand for CD40, gp39." in: The Journal of experimental medicine, Vol. 178, Issue 5, pp. 1567-75, 1993 (PubMed).

Roy, Waldschmidt, Aruffo, Ledbetter, Noelle: "The regulation of the expression of gp39, the CD40 ligand, on normal and cloned CD4+ T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 5, pp. 2497-510, 1993 (PubMed).

Noelle, Roy, Shepherd, Stamenkovic, Ledbetter, Aruffo: "A 39-kDa protein on activated helper T cells binds CD40 and transduces the signal for cognate activation of B cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 14, pp. 6550-4, 1992 (PubMed).

Hollenbaugh, Grosmaire, Kullas, Chalupny, Braesch-Andersen, Noelle, Stamenkovic, Ledbetter, Aruffo: "The human T cell antigen gp39, a member of the TNF gene family, is a ligand for the CD40 receptor: expression of a soluble form of gp39 with B cell co-stimulatory activity." in: The EMBO journal, Vol. 11, Issue 12, pp. 4313-21, 1992 (PubMed).

Armitage, Fanslow, Strockbine, Sato, Clifford, Macduff, Anderson, Gimpel, Davis-Smith, Maliszewski: "Molecular and biological characterization of a murine ligand for CD40." in: Nature, Vol. 357, Issue 6373, pp. 80-2, 1992 (PubMed).