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CD90 antibody (THY1)

Details for Product anti-THY1 Antibody No. ABIN371033, Supplier: Login to see
Antigen
  • CD90
  • CD7
  • T25
  • Thy-1
  • Thy-1.2
  • Thy1.1
  • Thy1.2
Reactivity
Mouse (Murine)
277
253
130
27
26
25
17
17
10
5
4
4
3
2
2
2
Host
Rat
299
196
100
16
Clonality (Clone)
Monoclonal ()
Conjugate
This CD90 antibody is un-conjugated
81
68
58
34
7
5
5
5
5
5
4
4
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
431
147
104
97
74
60
37
33
30
29
13
10
9
9
5
4
3
2
2
1
1
1
1
Supplier
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Clone 30-H12
Isotype IgG2b
Specificity This antibody recognises CD90.2/Thy-1.2 alloantigen (Mr. 18 kDa). It does not cross react with Mouse and Rat strains bearing the Thy-1.1 alloantigen (e.g., AKR/J,PL). (Ref.2-5).
Characteristics Synonyms: Thy1.2, Thy-1.2, CD90, Thy-1, Thy-1 membrane glycoprotein
Purification Purified
Alternative Name CD90 (THY1 Antibody Abstract)
Background CD90/Thy-1 is a GPI-anchored molecule and one of the smallest members of the immunoglobulin superfamily, consisting of a single V-set domain. (Ref.1-3) The Thy-1.2 alloantigen is expressed on all thymocytes, peripheral T lymphocytes, and some intraepithelial T cells of most mouse strains. It is also expressed in the brain and at varying levels on other non-lymphoid tissues.Synonyms: CD90, Thy-1, Thy-1 membrane glycoprotein, Thy-1.2, Thy1.2
Gene ID 21838
UniProt P01831
Research Area CD Antigens, Surface Receptors of Immune Cells, Experimental Models
Pathways
Application Notes Flow Cytometry: < / = 1 μg/10^6 cells. (Ref.4,5)Immunohistochemistry on Frozen Sections. (Ref.6)In vivo and in vitro Depletion of T Cells. (Ref.7,8)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer 100 mM Borate Buffered Saline, pH 8.2. No preservatives or amine-containing buffer salts added.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months
Background publications Williams, Gagnon: "Neuronal cell Thy-1 glycoprotein: homology with immunoglobulin." in: Science (New York, N.Y.), Vol. 216, Issue 4547, pp. 696-703, 1982 (PubMed).

Ledbetter, Rouse, Micklem et al.: "T cell subsets defined by expression of Lyt-1,2,3 and Thy-1 antigens. Two-parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views." in: The Journal of experimental medicine, Vol. 152, Issue 2, pp. 280-95, 1980 (PubMed).

Homans, Ferguson, Dwek et al.: "Complete structure of the glycosyl phosphatidylinositol membrane anchor of rat brain Thy-1 glycoprotein." in: Nature, Vol. 333, Issue 6170, pp. 269-72, 1988 (PubMed).

Ledbetter, Herzenberg: "Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens." in: Immunological reviews, Vol. 47, pp. 63-90, 1980 (PubMed).