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CD86 antibody

CD86 Reactivity: Mouse FACS, IP, IHC (fro), Func Host: Rat Monoclonal 2D10 unconjugated
Catalog No. ABIN371185
  • Target See all CD86 Antibodies
    CD86
    Reactivity
    • 139
    • 116
    • 50
    • 20
    • 10
    • 7
    • 5
    • 4
    • 3
    • 3
    • 2
    Mouse
    Host
    • 79
    • 69
    • 67
    Rat
    Clonality
    • 152
    • 60
    • 1
    Monoclonal
    Conjugate
    • 86
    • 23
    • 18
    • 14
    • 13
    • 5
    • 5
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This CD86 antibody is un-conjugated
    Application
    • 145
    • 97
    • 44
    • 41
    • 33
    • 31
    • 30
    • 23
    • 16
    • 16
    • 15
    • 14
    • 13
    • 11
    • 5
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)
    Specificity
    This antibody recognises CD86, the B7-2 costimulatory molecule and a ligand for CD28 and CD152.
    Characteristics
    Synonyms: CD28LG2, B70, FUN-1, BU63, T-lymphocyte activation antigen CD86, Activation B7-2antigen, CTLA-4 counter-receptor B7.2
    Purification
    Purified
    Clone
    2D10
    Isotype
    IgG2b
    Top Product
    Discover our top product CD86 Primary Antibody
  • Application Notes
    Flow Cytometry: Identification and enumeration of CD86+ cells. (Ref.1,3,4,9)Immunoprecipitation (Ref.3)Immunohistochemistry on Frozen Sections: Identification of CD86+ cells. (Ref.8,9)In vivo and in vitro blocking assays. (Ref.1,2,8,9)
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    0.5 mg/mL
    Buffer
    100 mM Borate Buffered Saline, pH 8.2. No preservatives or amine-containing buffer salts added.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
    Shelf life: one year from despatch.
    Expiry Date
    12 months
  • Target
    CD86
    Alternative Name
    CD86 (CD86 Products)
    Synonyms
    B7-2 antibody, B7.2 antibody, B70 antibody, CD28LG2 antibody, LAB72 antibody, B7 antibody, CLS1 antibody, Cd28l2 antibody, ETC-1 antibody, Ly-58 antibody, Ly58 antibody, MB7 antibody, MB7-2 antibody, TS/A-2 antibody, CD86 molecule antibody, CD86 antigen antibody, integrin subunit alpha 2b antibody, CD86 antibody, Cd86 antibody, Itga2b antibody
    Background
    CD86, also known as B72, is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes. CD86 expression is rapidly upregulated by B cell specific stimuli with peak expression at 18 to 42 hours after stimulation. CD86, along with CD80/B71, is an important accessory molecule in T cell costimulation via its interaction with CD28 and CD152/CTLA4. Since CD86 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response. It is also found on malignant Hodgkin and Reed Sternberg (HRS) cells in Hodgkin's disease. CD86 interacts with HHV8 (KSHV) MIR2 protein.Synonyms: Activation B7-2 antigen, B7.2, B70, BU63, CD28LG2, CTLA-4 counter-receptor B7.2, FUN-1, T-lymphocyte activation antigen CD86
    Gene ID
    12524
    UniProt
    P42082
    Pathways
    TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation
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