Mouse anti-Human IgM (Chain mu) Antibody

Catalog No. ABIN371464

Product Details

Target: IgM

Binding Specificity: Chain mu

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Dot Blot (DB), Enzyme Immunoassay (EIA)

Endotoxin Level: Low Endotoxin

Clone: UHB

Isotype: IgG3

Application Details

Application Notes: Indirect Immunofluorescent staining of IgM+ human B lymphocytes. ELISA. Western blotting. Dot- and Slot-Immunoblotting. Immunohistochemistry on Frozen Sections. Immunocytochemistry.

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at -20 °C.

Target Details

Target: IgM

Abstract: IgM Products

Target Type: Antibody

Background: IgM normally constitutes about 10 % of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and is largely confined to plasma due to it's large size. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells. Due to it's high valency IgM is more efficient than other isotypes is binding antigens with repeating epitopes (virus particles and red blood cells) and is more efficient than IgG in activiating the complement pathway. The gene for the mu constant region contains four domains separated by short intervening sequences. IgM measurement yields information about the body's immediate resistance and response to infection as well as information related to specific diseases. Decreased levels are associated with immune deficiency states, hereditary deficiencies, and myeloma. Increased levels can be associated with Waldenstrom's macroglobulinemia, chronic infection and hepatocellular disease.Synonyms: Human Immunoglobulin M