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FAS antibody

FAS Reactivity: Human FACS, IP, IHC (fro), Func Host: Mouse Monoclonal DX3 unconjugated
Catalog No. ABIN371584
  • Target See all FAS Antibodies
    FAS (TNF Receptor Superfamily, Member 6 (FAS))
    Reactivity
    • 214
    • 67
    • 62
    • 19
    • 6
    • 4
    • 2
    • 2
    • 1
    • 1
    Human
    Host
    • 159
    • 89
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    Mouse
    Clonality
    • 156
    • 100
    • 1
    Monoclonal
    Conjugate
    • 124
    • 27
    • 25
    • 12
    • 10
    • 7
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This FAS antibody is un-conjugated
    Application
    • 165
    • 117
    • 74
    • 65
    • 36
    • 35
    • 31
    • 26
    • 26
    • 20
    • 19
    • 9
    • 8
    • 6
    • 5
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)
    Specificity
    This antibody recognizes CD95/Fas/Apo-1. Crosslinking of CD95 by the Monoclonal antibodies DX2 and DX3 delivers an apoptotic signal to Fas-sensitive cells, indicating that these monoclonal antibodies recognize a functional epitope of CD95. (Ref.1-5)
    Characteristics
    Synonyms: FASLG receptor, Apo-1 antigen, APT1, FAS1, TNFRSF6, Tumor necrosis factor receptorsuperfamily member 6
    Purification
    Purified
    Clone
    DX3
    Isotype
    IgG2a
    Top Product
    Discover our top product FAS Primary Antibody
  • Application Notes
    Flow Cytometry: < / = 1 μg/10^6 cells. Immunohistochemistry on Frozen Sections. Immunoprecipitation. In vitro induction of apoptosis.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    0.1 mg/mL
    Buffer
    100 mM Borate Buffered Saline, pH 8.2. No preservatives or amine-containing buffer salts added.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
    Shelf life: one year from despatch.
    Expiry Date
    12 months
  • Target
    FAS (TNF Receptor Superfamily, Member 6 (FAS))
    Alternative Name
    CD95 / FAS (FAS Products)
    Synonyms
    ALPS1A antibody, APO-1 antibody, APT1 antibody, CD95 antibody, FAS1 antibody, FASTM antibody, TNFRSF6 antibody, AI196731 antibody, APO1 antibody, TNFR6 antibody, Tnfrsf6 antibody, lpr antibody, Fas cell surface death receptor antibody, Fas (TNF receptor superfamily member 6) antibody, FAS antibody, Fas antibody, fas antibody
    Background
    CD95, also known as FAS or APO1, is a 36 kDa cell surface type I membrane glycoprotein with an apparent molecular weight of 44 kDa on SDS PAGE. CD95 is a member of the TNF receptor family, which includes TNFR1, TNFR2, CD27, CD30 and CD40. Binding of CD95 Ligand to CD95 or crosslinking of CD95 by anti CD95 monoclonal antibodies leads to apoptosis of CD95 expressing cells. CD95 belongs to a subgroup of family members that have a death domain (DD) which contains 70 amino acids near the carboxyl terminal region of the molecule. The binding of adaptor molecules to this DD is responsible for transmitting the death signal for apoptosis. Stimulation of CD95 results in aggregation of its DD, leading to the recruitment of FADD and caspase 8 that together with the receptor form the death inducing signaling complex (DISC). CD95/CD95L is involved in the peripheral deletion of activated mature T cells at the end of the immune response and defects in this pathway predispose to autoimmune disorders. CD95 is also involved in killing of targets such as virus infected cells or cancer cells and killing of inflammatory cells at immune privileged sites.Synonyms: APT1, Apo-1 antigen, FAS1, FASLG receptor, TNFRSF6, Tumor necrosis factor receptor superfamily member 6
    Gene ID
    355
    UniProt
    P25445
    Pathways
    p53 Signaling, Apoptosis, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity
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