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Actin antibody
| Antigen | Actin |
| Synonyms |
A, Ac5C, Act, Actin, Actin/BAP47, Actin5C, BAP47, M32055, T11, act 5C, act5C, actin-5C, anon-EST:fe2D2, beta-actin/Bap47, cyt5C, l(1)G0009, l(1)G0010, l(1)G0025, l(1)G0079, l(1)G0117, l(1)G0177, l(1)G ...
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| Clonality | Monoclonal (C4) |
| Host |
 Alternatives Mouse |
| Application |
Alternatives Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB)
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| Catalog no. | ABIN371822 |
| Quantity | 100 µl (Variants) |
| Price | 290.00 $ Plus shipping costs $35.00 |
| Shipping to |
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| Availability | Ships within 7 to 10 Business Days |
Additional Information
| Alternative name | Actin (pan) |
| Immunogen | Chicken gizzard Actin. |
| Isotype | IgG1 (Matching secondary antibodies) |
| Clone | C4 |
| Description | Actin is the most abundant protein found in virtually all eukaryotic cells. It is also one ofthe most highly-conserved proteins, differing by no more than 20% in species as diverseas algae and humans, making it an excellent choice for use as a loading control. It is themonomeric subunit of microfilaments, one of the three major components of thecytoskeleton, and of thin filaments, which are part of the contractile apparatus in musclecells. Thus, actin participates in many important cellular functions, including musclecontraction, cell motility, cell division and cytokinesis, vesicle and organelle movement,cell signaling, and the establishment and maintenance of cell junctions and cell shape. |
| Specificity | This antibody is specific for the ~42 kDa actin protein in lysates from skeletal, cardiac,gizzard and aorta tissues. Reacts with all Actin isoforms. The antibody reacts with all known Actins from Yeast to Man. Species Reactivity: Tested: Xenopus, Drosophila, Zebrafish, Most mammals, Some invertebrates |
| Synonyms | A, Ac5C, Act, Actin, Actin/BAP47, Actin5C, BAP47, M32055, T11, act 5C, act5C, actin-5C, anon-EST:fe2D2, beta-actin/Bap47, cyt5C, l(1)G0009, l(1)G0010, l(1)G0025, l(1)G0079, l(1)G0117, l(1)G0177, l(1)G0245, l(1)G0330, l(1)G0420, l(1)G0486, DmelCG4027, CG4027, Act57A, Act57b, BEST:RE38067, C, CG30294, M, T10, act 57A, anon-EST:CL2c12, DmelCG10067, CG10067, 143060_f_at, D, act 79B, act79B, mus79B, DmelCG7478, CG7478, Act(88F), Act88-F, E, Ifm(3)7, act 88F, act88F, rsd, DmelCG5178, CG5178, act 87E, act87E, mus87E, DmelCG18290, CG18290, ACTIN, ACTIN-12, actin, dsim_GLEANR_2829, DsimGD19025, GD19025, Act88F, AFFX-Dros-ACTIN_M_r_at, Act42a, act 42A, DmelCG12051, CG12051 |
Application Details
| Application Notes | Immunohistochemistry: 1/500. Western blotting: 1/5,000. Immunofluorescence: 1/500. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. |
| Purification | Ascites |
| Storage | Store the antibody undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| Research Area | Extracellular Matrix |
| Restrictions | For Research Use only |
Alternatives
Alternatives for antigen "Actin", type "Antibodies"
| Hosts | Mouse (54), Rabbit (22), Sheep (1) |
| Reactivities | Human (48), Chicken (20), Rat (Rattus) (18), Cow (Bovine) (12), Mouse (Murine) (9), Rabbit (9), Guinea Pig (6), Sheep (Ovine) (5), Snake (5), Carp (4), Frog (4), Dog (Canine) (2), Pig (Porcine) (2), Xenopus laevis (2), All Species (1), Cat (Feline) (1), Horse (Equine) (1), Plant (1), Primates (1) |
| Applications | Western Blotting (WB) (48), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (39), Immunohistochemistry (Frozen Sections) (IHC (fro)) (21), Immunofluorescence (IF) (20), Immunohistochemistry (IHC) (18), Immunoprecipitation (IP) (16), ELISA (14), Immunocytochemistry (ICC) (6), Enzyme Immunoassay (EIA) (3), Flow Cytometry (FACS) (2), Immunoelectron Microscopy (EM) (1), Radioimmunoassay (RIA) (1) |
| Conjugates | Biotin (5), Cy3 (1), FITC (1) |
| Epitopes | pan (5), Ab-55/53 (1), Asp244 (1), pTyr55/53 (1) |
