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Fibrillarin antibody

FBL Reactivity: Human, Rat, Drosophila melanogaster, C. elegans, Plant, Schizosaccharomyces pombe WB, IF, IHC (fro) Host: Mouse Monoclonal 38F3 unconjugated
Catalog No. ABIN371824
  • Target See all Fibrillarin (FBL) Antibodies
    Fibrillarin (FBL)
    Reactivity
    • 45
    • 23
    • 21
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Human, Rat, Drosophila melanogaster, C. elegans, Plant, Schizosaccharomyces pombe
    Host
    • 39
    • 10
    • 1
    Mouse
    Clonality
    • 38
    • 12
    Monoclonal
    Conjugate
    • 35
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Fibrillarin antibody is un-conjugated
    Application
    • 48
    • 12
    • 11
    • 9
    • 8
    • 7
    • 5
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Specificity
    Specific for the ~34 kDa Fibrillarin /Nop1p protein. This antibody is becoming widely used as a convenient marker for nucleoli in a wide variety of species (e.g. 4-6).
    Cross-Reactivity (Details)
    Species reactivity (expected):Mammals.
    Species reactivity (tested):Human, Rat, Plant, Drosophila, C. elegans and S. pombe.
    Characteristics
    Synonyms: FIB1, FLRN, Fibrillarin, RNU3IP1, NOP1, LOT3, Nucleolar protein 1, Nucleolar Marker, rRNA2'-O-methyltransferase fibrillarin, U3 small nucleolar RNA-associated protein NOP1
    Purification
    Ig Fraction
    Immunogen
    Yeast nuclear preparations.
    Clone
    38F3
    Isotype
    IgG1
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    Discover our top product FBL Primary Antibody
  • Application Notes
    Western Blot: 1/1,000. Immunofluorescence: 1/500. Immunohistochemistry.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Store the antibody undiluted (in aliquots) at -20 °C.
    Shelf life: one year from despatch.
    Expiry Date
    12 months
  • Target
    Fibrillarin (FBL)
    Alternative Name
    FBL (FBL Products)
    Synonyms
    FIB antibody, FLRN antibody, RNU3IP1 antibody, wu:fb37g12 antibody, zgc:56145 antibody, zgc:77130 antibody, FBL antibody, AL022665 antibody, CG9888 antibody, Dmel\\CG9888 antibody, Fibri antibody, GCR-6 antibody, GCR6 antibody, Pen59C5 antibody, fib antibody, pen59C5 antibody, MGC76139 antibody, fbl antibody, fibrillarin antibody, Fibrillarin antibody, putative fibrillarin antibody, fibrillarin S homeolog antibody, FBL antibody, fbl antibody, Fbl antibody, Fib antibody, fib1 antibody, LMJF_19_0100 antibody, fbl.S antibody
    Background
    Nop1p was originally identified as a nucleolar protein of bakers yeast, Saccharomyces cerevisiae. The Nop1p protein is 327 amino acids in size (34.5 kDa), is essential for yeast viability, and is localized in the nucleoli (1). The systematic name for S. cerevisiae Nop1 is YDL014W, and it is now known to be part of the small subunit processome complex, involved in the processing of pre-18S ribosomal RNA. Nop1p is the yeast homologue of a protein found in all eukaryotes and archea generally called fibrillarin (2). Fibrillarin/Nop1p is extraordinarily conserved, so that the yeast and human proteins are 67 % identical, and the human protein can functionally replace the yeast protein. Patients with the autoimmune disease scleroderma often have strong circulating autoantibodies to a ~34 kDa protein which was subsequently found to be fibrillarin. Recent studies show that knock-out of the fibrillarin gene in mice results in embryonic lethality, although mice with only one functional fibrillarin/Nop1p gene were viable (3).Synonyms: FIB1, FLRN, Fibrillarin, LOT3, NOP1, Nucleolar Marker, Nucleolar protein 1, RNU3IP1, U3 small nucleolar RNA-associated protein NOP1, rRNA 2'-O-methyltransferase fibrillarin
    Gene ID
    851548
    UniProt
    P15646
    Pathways
    Ribonucleoside Biosynthetic Process
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