Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) antibody

Details for Product No. ABIN371825
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Antigen
Synonyms
CG8893, Dmel\\CG8893, GA3PDH, GADPH, GAP, GAPDH, GAPDH II, GAPDH2, Gapd, Gapdh, Gapdh-2, Gapdh13F, GAPD, g3pd, gapd, GB14798, GAPC-2, GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE C-2, T6J4.17, T6J4_17, gl ... show more
CG8893, Dmel\\CG8893, GA3PDH, GADPH, GAP, GAPDH, GAPDH II, GAPDH2, Gapd, Gapdh, Gapdh-2, Gapdh13F, GAPD, g3pd, gapd, GB14798, GAPC-2, GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE C-2, T6J4.17, T6J4_17, glyceraldehyde-3-phosphate dehydrogenase C2, BEST:GH12586, CG12055, Dmel\\CG12055, GAPDH I, GAPDH-1, GAPDH1, GAPDHI, Gapdh-1, Gapdh43E, gadph, gapdh, gapdh-1, gh12586, KNC-NDS6, G3PD, cb609, mg:bb02e05, wu:fb33a10, wu:ft80f05, G3PDH show less
Reactivity
Cow (Bovine), Chicken, Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
(400), (217), (194), (95), (37), (29), (27), (27), (17), (17), (16), (13), (12), (12), (9), (9), (8), (8), (7), (6), (6), (6), (4), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Host
Mouse
(276), (190), (34), (18), (17)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(31), (28), (12), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (2), (2), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
(448), (226), (125), (78), (76), (73), (49), (27), (16), (10), (5), (4), (4), (3), (3), (2), (2), (2), (2), (1), (1)
Pubmed 5 references available
Quantity 0.1 mL
Shipping to United States (Change)
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Catalog No. ABIN371825
335.50 $
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Immunogen Extensively purified pig GAPDH.
Clone 1D4
Isotype IgM
Characteristics Synonyms: GAPD, CDABP0047, Loading control, Glyceraldehyde-3-Phosphate Dehydrogenase
Purification Supernatant
Alternative Name GAPDH
Background Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) is a metabolic enzyme responsiblefor catalyzing one step in the glycolytic pathway, the reversible oxidative phosphorylationof glyceraldehyde 3-phosphate. Because GAPDH is a protein expressed in large amountsand which is required at all times for important house keeping functions, levels ofGAPDH mRNA are often measured and used as standards in studies of mRNA expression. Increasingly, scientists are making use of specific antibodies to GAPDH in comparablestudies of levels of protein expression. This antibody can be used as a loading control forwestern blotting experiments, allowing comparison between the level of this protein andothers in a cell or tissue. Apart from a role in glycolysis, GAPDH may have other roles suchas in the activation of transcription (1). GAPDH is reported to bind to a variety of otherproteins, including the amyloid precursor protein, mutations in which cause some forms ofAlzheimer's disease, and the polyglutamine tracts of Huntingtin, the protein productaberrant forms of which are causative of Huntington's disease (2,3). Associations withactin and tubulin have also been reported. The protein may also have a role in theregulation of apoptosis, and interestingly migrates from the cytoplasm into the nucleuswhen cells become apoptotic (4).
UniProt P04406
Research Area Cancer, Metabolism, Alzheimer's Disease, Enzymes
Application Notes Western Blot: 1/1,000. Immunofluorescence: 1/100. Immunohistochemistry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Storage -20 °C
Storage Comment Store the antibody undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Expiry Date 12 months
Supplier Images
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) antibody anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) antibody
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) antibody (2) anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) antibody (Image 2)
Product cited in: Fehrholz, Kendl, Prifert et al.: "The innate antiviral factor APOBEC3G targets replication of measles, mumps and respiratory syncytial viruses." in: The Journal of general virology, Vol. 93, Issue Pt 3, pp. 565-76, 2012 (PubMed).

Background publications Morgenegg, Winkler, Hübscher et al.: "Glyceraldehyde-3-phosphate dehydrogenase is a nonhistone protein and a possible activator of transcription in neurons." in: Journal of neurochemistry, Vol. 47, Issue 1, pp. 54-62, 1986 (PubMed).

Schulze, Schuler, Stüber et al.: "Rat brain glyceraldehyde-3-phosphate dehydrogenase interacts with the recombinant cytoplasmic domain of Alzheimer's beta-amyloid precursor protein." in: Journal of neurochemistry, Vol. 60, Issue 5, pp. 1915-22, 1993 (PubMed).

Burke, Enghild, Martin et al.: "Huntingtin and DRPLA proteins selectively interact with the enzyme GAPDH." in: Nature medicine, Vol. 2, Issue 3, pp. 347-50, 1996 (PubMed).

Dastoor, Dreyer: "Potential role of nuclear translocation of glyceraldehyde-3-phosphate dehydrogenase in apoptosis and oxidative stress." in: Journal of cell science, Vol. 114, Issue Pt 9, pp. 1643-53, 2001 (PubMed).

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