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GAPDH antibody (Glyceraldehyde-3-Phosphate Dehydrogenase)

Details for Product anti-GAPDH Antibody No. ABIN371825, Supplier: Login to see
Antigen
  • CG8893
  • Dmel\\CG8893
  • GA3PDH
  • GADPH
  • GAP
  • GAPDH
  • GAPDH II
  • GAPDH2
  • Gapd
  • Gapdh
  • Gapdh-2
  • Gapdh13F
  • GAPD
  • g3pd
  • gapd
  • GB14798
  • GAPC-2
  • GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE C-2
  • T6J4.17
  • T6J4_17
  • glyceraldehyde-3-phosphate dehydrogenase C2
  • BEST:GH12586
  • CG12055
  • Dmel\\CG12055
  • GAPDH I
  • GAPDH-1
  • GAPDH1
  • GAPDHI
  • Gapdh-1
  • Gapdh43E
  • gadph
  • gapdh
  • gapdh-1
  • gh12586
  • KNC-NDS6
  • G3PD
  • cb609
  • mg:bb02e05
  • wu:fb33a10
  • wu:ft80f05
  • G3PDH
Reactivity
Cow (Bovine), Chicken, Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
552
219
192
116
56
50
44
30
29
25
18
18
17
13
12
11
8
8
8
7
6
6
6
6
5
5
4
4
4
4
4
3
3
3
2
1
1
1
1
1
1
1
1
1
1
Host
Mouse
339
240
60
31
21
Clonality (Clone)
Monoclonal ()
Conjugate
This GAPDH antibody is un-conjugated
42
40
18
6
6
6
5
4
4
4
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB)
604
283
156
129
90
64
48
26
22
17
10
10
7
6
5
5
4
4
2
2
2
2
2
1
1
1
1
1
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Immunogen Extensively purified pig GAPDH.
Clone 1D4
Isotype IgM
Specificity This antibody is specific for the ~38k Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) protein.
Cross-Reactivity (Details) Species reactivity (expected):Avian and Bovine.
Species reactivity (tested):Human, Bovine, Chicken, Porcine, Mouse and Rat.
Characteristics Synonyms: GAPD, CDABP0047, Loading control, Glyceraldehyde-3-Phosphate Dehydrogenase
Purification Supernatant
Alternative Name GAPDH (GAPDH Antibody Abstract)
Background Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) is a metabolic enzyme responsible for catalyzing one step in the glycolytic pathway, the reversible oxidative phosphorylation of glyceraldehyde 3-phosphate. Because GAPDH is a protein expressed in large amounts and which is required at all times for important "house keeping" functions, levels of GAPDH mRNA are often measured and used as standards in studies of mRNA expression. Increasingly, scientists are making use of specific antibodies to GAPDH in comparable studies of levels of protein expression. This antibody can be used as a loading control for western blotting experiments, allowing comparison between the level of this protein and others in a cell or tissue. Apart from a role in glycolysis, GAPDH may have other roles such as in the activation of transcription (1). GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease, and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease (2,3). Associations with actin and tubulin have also been reported. The protein may also have a role in the regulation of apoptosis, and interestingly migrates from the cytoplasm into the nucleus when cells become apoptotic (4).Synonyms: CDABP0047, GAPD, Glyceraldehyde-3-Phosphate Dehydrogenase
Gene ID 2597
UniProt P04406
Research Area Cancer, Metabolism, Alzheimer's Disease, Enzymes
Pathways
Application Notes Western Blot: 1/1,000. Immunofluorescence: 1/100. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Storage -20 °C
Storage Comment Store the antibody undiluted (in aliquots) at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months
Supplier Images
Western Blotting (WB) image for anti-GAPDH antibody (Glyceraldehyde-3-Phosphate Dehydrogenase) (ABIN371825) anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) antibody
Immunofluorescence (IF) image for anti-GAPDH antibody (Glyceraldehyde-3-Phosphate Dehydrogenase) (ABIN371825) anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) antibody (Image 2)
Product cited in: Fehrholz, Kendl, Prifert et al.: "The innate antiviral factor APOBEC3G targets replication of measles, mumps and respiratory syncytial viruses." in: The Journal of general virology, Vol. 93, Issue Pt 3, pp. 565-76, 2012 (PubMed).

Background publications Dastoor, Dreyer: "Potential role of nuclear translocation of glyceraldehyde-3-phosphate dehydrogenase in apoptosis and oxidative stress." in: Journal of cell science, Vol. 114, Issue Pt 9, pp. 1643-53, 2001 (PubMed).

Burke, Enghild, Martin et al.: "Huntingtin and DRPLA proteins selectively interact with the enzyme GAPDH." in: Nature medicine, Vol. 2, Issue 3, pp. 347-50, 1996 (PubMed).

Schulze, Schuler, Stüber et al.: "Rat brain glyceraldehyde-3-phosphate dehydrogenase interacts with the recombinant cytoplasmic domain of Alzheimer's beta-amyloid precursor protein." in: Journal of neurochemistry, Vol. 60, Issue 5, pp. 1915-22, 1993 (PubMed).

Morgenegg, Winkler, Hübscher et al.: "Glyceraldehyde-3-phosphate dehydrogenase is a nonhistone protein and a possible activator of transcription in neurons." in: Journal of neurochemistry, Vol. 47, Issue 1, pp. 54-62, 1986 (PubMed).