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UCHL1 antibody (Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase))

Details for Product anti-UCHL1 Antibody No. ABIN371848, Supplier: Log in to see
Antigen
  • AW822034
  • C88048
  • cb358
  • gad
  • MGC132191
  • park5
  • PARK5
  • PGP9.5
  • PGP 9.5
  • pgp9.5
  • PGP95
  • R75593
  • uch-l1
  • UCH-L1
  • Uch-L1
  • UCHL-1
  • UCHL1
  • uchl1
  • wu:fc55h08
Reactivity
Cow (Bovine), Human, Mammalian, Mouse (Murine), Rat (Rattus)
490
244
224
116
111
70
58
49
49
49
7
7
3
2
1
Host
Mouse
256
202
34
18
7
4
Clonality (Clone)
Monoclonal ()
Conjugate
This UCHL1 antibody is un-conjugated
19
18
16
14
10
10
9
9
9
9
9
7
7
7
7
7
7
7
7
7
1
1
1
1
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB)
416
229
176
128
105
71
67
13
11
9
6
5
3
3
Supplier
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Immunogen Recombinant full length human UCHL1 purified from E. coli.
Clone BH7
Isotype IgG1
Specificity AM08273SU-N is specific for the ~24 kDa UCHL1 protein.
Cross-Reactivity (Details) Species reactivity (expected):most Mammalian.
Species reactivity (tested):Bovine, Human, Mouse and Rat.
Characteristics Synonyms: UCH-L1, PGP 9.5, Ubiquitin thioesterase L1, Neuron cytoplasmic protein 9.5, Ubiquitincarboxyl-terminal hydrolase isozyme L1
Purification Supernatant
Alternative Name UCHL1 / PGP9.5 (UCHL1 Antibody Abstract)
Background Ubiquitin C-terminal hydrolase 1 (UCHL1) is also known as ubiquitin carboxyl esterase L1, ubiquitin thiolesterase, neuron-specific protein PGP9.5 and Park5. It was originally identified as a major component of the neuronal cytoplasm from 2-dimensional gel analysis of brain tissues, and was given the name PGP9.5 (1). It was later found that ubiquitin C-terminal hydrolase enzyme activity was associated with the PGP9.5 protein (2). The ubiquitin C-terminal hydrolases cleave ubiquitin from other molecules. Regulation of the ubiquitin pathway is very important and many disease states are associated with defects in this pathway. Genetic knockout of UCHL1 in mice results in a motor neuron degeneration similar to the spontaneous gracile axonal dystrophy (gad) mutant mice (3). Point mutations in the UCHL1 gene are associated with some forms of human Parkinson's disease (4). Since UCHL1 is heavily expressed in neurons, it is released in large amounts following injury or degeneration, so the detection of UCHL1 in CSF and other bodily fluids can be used as a biomarker.Synonyms: Neuron cytoplasmic protein 9.5, PGP 9.5, UCH-L1, Ubiquitin carboxyl-terminal hydrolase isozyme L1, Ubiquitin thioesterase L1
Gene ID 7345
UniProt P09936
Research Area Tags/Labels, Cell/Tissue Markers
Application Notes Western Blot: 1/10,000. Immunohistochemistry. Immunofluorescence: 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Storage -20 °C
Storage Comment Store the antibody undiluted (in aliquots) at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months
Supplier Images
Western Blotting (WB) image for anti-UCHL1 antibody (Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase)) (ABIN371848) anti-Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1) antibody
Immunofluorescence (IF) image for anti-UCHL1 antibody (Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase)) (ABIN371848) anti-Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1) antibody (Image 2)
Background publications Liu, Fallon, Lashuel, Liu, Lansbury: "The UCH-L1 gene encodes two opposing enzymatic activities that affect alpha-synuclein degradation and Parkinson's disease susceptibility." in: Cell, Vol. 111, Issue 2, pp. 209-18, 2002 (PubMed).

Kurihara, Kikuchi, Wada, Tilghman: "Loss of Uch-L1 and Uch-L3 leads to neurodegeneration, posterior paralysis and dysphagia." in: Human molecular genetics, Vol. 10, Issue 18, pp. 1963-70, 2001 (PubMed).

Wilkinson, Lee, Deshpande, Duerksen-Hughes, Boss, Pohl: "The neuron-specific protein PGP 9.5 is a ubiquitin carboxyl-terminal hydrolase." in: Science (New York, N.Y.), Vol. 246, Issue 4930, pp. 670-3, 1989 (PubMed).

Doran, Jackson, Kynoch, Thompson: "Isolation of PGP 9.5, a new human neurone-specific protein detected by high-resolution two-dimensional electrophoresis." in: Journal of neurochemistry, Vol. 40, Issue 6, pp. 1542-7, 1983 (PubMed).