Blood Group Lewis A antibody
| Antigen |
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| Clonality |
Monoclonal (SPM522) |
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Host
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Reactivity
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Human (43), Mouse (Murine) (19), Rat (Rattus) (14), Dog (Canine) (10), Cow (Bovine) (9), Pig (Porcine) (7), Rabbit (4), Chicken (2), Horse (Equine) (2), Monkey (1), Zebrafish (Brachydanio rerio) (1)
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Application
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 Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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| Catalog no. |
ABIN372057 |
| Quantity |
0.1 ml (0,2 mg/ml) (Variants) |
| Price |
195.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Alternative name
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Blood Group Antigen Lewis a
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Immunogen
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Mucin isolated from an ovarian cyst
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Cross-Reactivity
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Human
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Isotype
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IgG1 (Matching secondary antibodies)
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Clone
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SPM522
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Description
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Lewis A is a blood group antigen consisting of Gal Beta1-3(Fuc alpha1-4) GlcNAc. Lewis A isfound to be present primarily on epithelial cells such as colon and kidneys. 2-4 in thetumors and dedifferentiated tissues, decrease of Lea antigen was observed.
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Specificity
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This antibody reacts to Lewis A. Species: Human. Others not tested.
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Application Notes
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Immunohistochemistry on paraffin sections: 1/200 for 30 min at RT, Staining offormalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10min followed by cooling at RT for 20 min. Positive control: Colon carcinomaOther applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Concentration
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0,2 mg/ml
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Purification
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Purified
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Buffer
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10mM PBS, pH 7.4 with BSA and Sodium Azide
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Storage
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Store the antibody undiluted at 2-8°C. DO NOT FREEZE! Shelf life: one year from despatch.
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Research Area
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Cell/Tissue Markers, Blood Group Antigens
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Restrictions
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For Research Use only
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Alternatives for antigen "Blood Group Lewis A", type "Antibodies"
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Hosts
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Rabbit (36), Mouse (11)
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Reactivities
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Human (43), Mouse (Murine) (19), Rat (Rattus) (14), Dog (Canine) (10), Cow (Bovine) (9), Pig (Porcine) (7), Rabbit (4), Chicken (2), Horse (Equine) (2), Monkey (1), Zebrafish (Brachydanio rerio) (1)
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Applications
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Flow Cytometry (FACS) (32), Immunofluorescence (IF) (30), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (12), Western Blotting (WB) (7), ELISA (6), Enzyme Immunoassay (EIA) (3), Immunoelectron Microscopy (IEM) (2), Immunohistochemistry (Frozen Sections) (IHC (fro)) (2), Immunohistochemistry (IHC) (1)
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Conjugates
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Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Biotin (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), HRP (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2), RBITC (2)
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