G Protein-Coupled Bile Acid Receptor 1 (GPBAR1) (AA 302-322) antibody

Antigen: G Protein-Coupled Bile Acid Receptor 1 (GPBAR1)

Synonyms: BG37, GPCR, TGR5, M-BAR, GPCR19, GPR131, MGC40597, GPBAR1, MGC152072, Tgr5, MGC84403, Gpbar1

Epitope: AA 302-322

Clonality: Polyclonal

Host: Rabbit

Reactivity: Chimpanzee

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN372269

Additional Information

Characteristics: Synonyms: G-protein coupled bile acid receptor 1, BG37, G-protein coupled receptor GPCR19, M-BAR,Membrane-type receptor for bile acids

Alternative name: GPBAR1 / TGR5

Swiss-Prot: Q8TDU6

Immunogen: Synthetic peptide (KLH conjugated) corresponding to a portion of the amino acids 300-350of Human BG37/TGR5.

Cross-Reactivity: Chimpanzee, Horse (Equine), Human, Monkey, Mouse (Murine)

Isotype: IgG  (Matching secondary antibodies)

Description: GPBAR1/TGR5 is a member of the G protein-coupled receptor family (GPCRs, or GPRs) whichcontain 7 transmembrane domains and transduce extracellular signals throughheterotrimeric G proteins. GPCR TGR5 is a receptor for bile acids. Bile acid-binding inducesits internalization, activation of extracellular signal-regulated kinase and intracellularcAMP production. It may be involved in the suppression of macrophage functions by bileacids.

Specificity: This antibody recognizes G Protein-Coupled Receptor BG37/TGR5 (GPBAR1). Species Reactivity: Tested: Human. Expected from sequence similarity: Chimpanzee, Horse, Monkey and Mouse.

Application Details

Application Notes: Western Blot: 2-4 µg/ml. Immunohistochemistry on Paraffin Sections: 10 µg/ml. This antibody was validated for use in immunohistochemistry on a panel of 21formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigenretrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides wereincubated with biotinylated secondary antibody, followed by alkalinephosphatase-streptavidin and chromogen. The stained slides were evaluated by apathologist to confirm staining specificity. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.5 mg/ml

Purification: Aff - Purified

Buffer: PBS containing 0.05% Sodium Azide as preservative and 0.2% Gelatin asstabilizer.

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Restrictions: For Research Use only