Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) (AA 590-640) antibody

Antigen: Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1)

Synonyms: ECGP, GP96, TRA1, GRP94, TA-3, Tra1, gp96, ERp99, Targ2, Tra-1, endoplasmin, Grp94, tra1, tra-1, fb61d09, wu:fb61d09, wu:fq25g01, Gp96, ppk98, ecgp, grp94, HSP90B1

Epitope: AA 590-640

Clonality: Polyclonal

Host: Rabbit

Reactivity: Cow (Bovine)

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN372279

Additional Information

Characteristics: Synonyms: Tumor rejection antigen 1, GRP94, Heat shock protein 90 kDa beta member 1, 94 kDaglucose-regulated protein

Alternative name: Endoplasmin / HSP90B1 / TRA1

Swiss-Prot: P14625

Immunogen: Synthetic peptide corresponding to a portion of the amino acids 590-640 of human GRP94.

Cross-Reactivity: Cow (Bovine), Chimpanzee, Chicken, Horse (Equine), Human, Monkey, Mouse (Murine), Rat (Rattus), Xenopus laevis

Isotype: IgG  (Matching secondary antibodies)

Description: Glucose regulated protein 94 (GRP 94) is a resident protein of the endoplasmic reticulum(ER) and is induced by the accumulation of unfolded proteins suggesting that it mightassociate transiently with a variety of newly synthesized secretory and membrane proteinsor permanently with mutant or defective proteins. The highly conserved sequenceLys-Asp-Glu-Leu (KDEL) is present at the C terminus of GRP 94 and other resident ERproteins including GRP 78 and protein disulfide isomerase (PDI). The presence of carboxyterminal KDEL appears to be necessary for retention and appears to be sufficient to reducethe secretion of proteins from the ER. This retention is reported to be mediated by a KDELreceptor. GRP 94 is also a low affinity, high capacity calcium binding protein, though it'srole, if any, in calcium regulation is not understood.

Specificity: This antibody recognizes Endoplasmin (HSP90B1/GRP94). Species Reactivity: Tested: Human. Expected from sequence similarity: Bovine, Chimpanzee, Chicken, Xenopus, Horse(Equine), Monkey, Mouse, Rat and Zebrafish.

Application Details

Application Notes: Immunohistochemistry on Paraffin Sections: 10 µg/ml. Western Blot: 0.5-2.0 µg/ml. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.5 mg/ml

Purification: Aff - Purified

Buffer: PBS containing 0.05% Sodium Azide as preservative and 0.2% Gelatin asstabilizer.

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Restrictions: For Research Use only