BUB1A (BUB1A) antibody

Antigen: BUB1A (BUB1A)

Synonyms: C86192, AI323730, AL022789, Ab2-098, zgc:92230, MGC127743

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN372471

Additional Information

Characteristics: Synonyms: BUB1L, Mitotic checkpoint serine/threonine-protein kinase BUB1

Alternative name: BUB1A

Swiss-Prot: O43683

Immunogen: Synthetic peptide corresponding to 19 amino acids from near the amino terminus of humanBub1.

Cross-Reactivity: Human, Mouse (Murine)

Isotype: IgG  (Matching secondary antibodies)

Description: Bub1 was initially identified as the mammalian homolog to the yeast protein through acDNA screen. Bub1 encodes a kinase involved in the formation of the spindle checkpoint,an important mechanism that ensures high fidelity mitotic chromosome segregation. It isthought to be required for assembly of a functional inner centromere, sister chromatidcohesion via targeting of the Shugoshim protein and metaphase congression. Bub1functions by phosphorylating cdc20, a member of the mitotic checkpoint complex andactivating the spindle checkpoint. A related protein kinase Bub3 interacts with Bub1 andtargets it to kinetochores prior to chromosome alignment. Mutations in bub1 have beenassociated with aneuploidy and several forms of cancer.

Specificity: This antibody recognizes Mitotic Checkpoint Kinase BUB1. Species Reactivity: Tested: Human. Expected from sequence similarity: Mouse.

Application Details

Application Notes: Immunohistochemistry on Paraffin Sections: 5 µg/ml. Western blot: 4 µg/ml. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml

Purification: Aff - Purified

Buffer: PBS containing 0.02% Sodium Azide as preservative.

Storage: Store the antibody undiluted at 2-8°C. Shelf life: one year from despatch.

Research Area: Cell Cycle

Restrictions: For Research Use only