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Details for Product No. ABIN372595

CAMK2A/B (pThr305) antibody

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Antigen
Epitope
»Alternatives pThr305
Reactivity
»Alternatives Cow (Bovine), Dog (Canine), Chicken, Human, Mouse (Murine), Xenopus laevis, Zebrafish (Danio rerio)
Host
»Alternatives Rabbit
Clonality Polyclonal
Application
»Alternatives Western Blotting (WB)
Pubmed 7 references available
Catalog no. ABIN372595
Quantity 0.1 mL
Price
324.50 $   Plus shipping costs $45.00
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Availability Will be delivered in 6 to 8 Business Days
Immunogen Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr305 found in rat brain CaM Kinase II.
Isotype IgG
Specificity Specific for the ~50k Alpha-CaM Kinase II and the ~60k Beta-CaM Kinase II proteins phosphorylated at Thr305. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. Note: The antibody should also recognize the Delta CaM kinase II subunit based on sequence homology with the antigen.
Purification Serum
Background Ca2+/Calmodulin-Dependent Protein Kinase II (CaM Kinase II) is a multi-functional calcium and calmodulin-dependent protein kinase that mediates cellular responses to a wide variety of intercellular signals (Kennedy, 1998, Schulman and Hanson, 1993). CaM Kinase II has been shown to regulate diverse cellular functions including synaptic plasticity, neurotransmitter synthesis and release, gene expression, ion channel function, carbohydrate metabolism, cytoskeletal function, and Ca2+-homeostasis (Gleason et al., 2003, Soderling, 2000, Hudmon and Schulman, 2002). Phosphorylation of Thr286 on the kinase produces an autonomously active form of CaM Kinase II (Meng et al., 2003, Picciotto et al., 1993). Autophosphorylation of Thr305 inhibits the activity of CaM Kinase II. Phosphorylation at this site appears to reduce the association of CaM Kinase II with the PSD and reduce LTP and learning (Elgersma et al., 2002).
Alternate names: CAMK2A, CAMK2B, CAMKA, CaM kinase II subunit alpha, CaM kinase II subunit beta, CaM- kinase II alpha chain, CaM-kinase II beta chain, CaMK-II subunit alpha, CaMK-II subunit beta, Calcium/calmodulin-dependent protein kinase type II, KIAA0968
Gene ID 815
NCBI Accession NP_741960
UniProt Q9UQM7
Application Notes Western Blot: 1/1000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody undiluted (in aliquots) at-20°C.
Expiry Date 12 months
Background publications Picciotto, Czernik, Nairn: "Calcium/calmodulin-dependent protein kinase I. cDNA cloning and identification of autophosphorylation site." in: The Journal of biological chemistry, Vol. 268, Issue 35, pp. 26512-21, 1994 (PubMed).

Schulman, Hanson: "Multifunctional Ca2+/calmodulin-dependent protein kinase." in: Neurochemical research, Vol. 18, Issue 1, pp. 65-77, 1993 (PubMed).

Soderling: "CaM-kinases: modulators of synaptic plasticity." in: Current opinion in neurobiology, Vol. 10, Issue 3, pp. 375-80, 2000 (PubMed).

Hudmon, Schulman: "Neuronal CA2+/calmodulin-dependent protein kinase II: the role of structure and autoregulation in cellular function." in: Annual review of biochemistry, Vol. 71, pp. 473-510, 2002 (PubMed).

Elgersma, Fedorov, Ikonen et al.: "Inhibitory autophosphorylation of CaMKII controls PSD association, plasticity, and learning." in: Neuron, Vol. 36, Issue 3, pp. 493-505, 2002 (PubMed).

Gleason, Higashijima, Dallman et al.: "Translocation of CaM kinase II to synaptic sites in vivo." in: Nature neuroscience, Vol. 6, Issue 3, pp. 217-8, 2003 (PubMed).

Meng, Guo, Zhang et al.: "Autophosphorylated calcium/calmodulin-dependent protein kinase II alpha (CaMKII alpha) reversibly targets to and phosphorylates N-methyl-D-aspartate receptor subunit 2B (NR2B) in cerebral ischemia and reperfusion in hippocampus of rats." in: Brain research, Vol. 967, Issue 1-2, pp. 161-9, 2003 (PubMed).

Alternatives for antigen "CAMK2A/B", type "Antibodies"
Hosts (6)
Reactivities (6), (6), (6), (2)
Applications (6), (4), (4), (1)
Epitopes (4), (2)
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