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Characteristics
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Synonyms: CAMK2A/B, CAMKA, KIAA0968, CaMK-II-alpha, CaMK-II-beta,Calcium/calmodulin-dependent protein kinase type II
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Immunogen
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Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr305found in rat brain CaM Kinase II.
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Cross-Reactivity
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Cow (Bovine), Dog (Canine), Chicken, Human, Mouse (Murine), Xenopus laevis
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Isotype
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IgG (Matching secondary antibodies)
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Description
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Ca2+/Calmodulin-Dependent Protein Kinase II (CaM Kinase II) is a multi-functional calciumand calmodulin-dependent protein kinase that mediates cellular responses to a widevariety of intercellular signals (Kennedy, 1998, Schulman and Hanson, 1993). CaM Kinase IIhas been shown to regulate diverse cellular functions including synaptic plasticity,neurotransmitter synthesis and release, gene expression, ion channel function,carbohydrate metabolism, cytoskeletal function, and Ca2+-homeostasis (Gleason et al. ,2003, Soderling, 2000, Hudmon and Schulman, 2002). Phosphorylation of Thr286 on thekinase produces an autonomously active form of CaM Kinase II (Meng et al. , 2003,Picciotto et al. , 1993). Autophosphorylation of Thr305 inhibits the activity of CaM Kinase II. Phosphorylation at this site appears to reduce the association of CaM Kinase II with thePSD and reduce LTP and learning (Elgersma et al. , 2002).
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Specificity
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Specific for the ~50k Alpha-CaM Kinase II and the ~60k Beta-CaM Kinase II proteinsphosphorylated at Thr305. Immunolabeling is blocked by the phosphopeptide used as antigen but not by thecorresponding dephosphopeptide. Note: The antibody should also recognize the Delta CaM kinase II subunit based onsequence homology with the antigen. Species Reactivity: Tested: Rat. Expected from sequence similarity: Humnan, Bovine, Chicken, Canine, Mouse, Xenopusand Zebrafish.
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